Identification of Hexapeptides that Render C2 Myoblasts the Resistant to Menadione-induced Cell Death

다국어 초록 (Multilingual Abstract)

Menadione induced cell death in cultured C2myoblasts. By screening synthetic peptide libraries composedof random sequence of hexapeptides, we identified thehexa-peptides pool of (Ala/Ile)-(Ile/Met)-Val-Ile-Asp-(Met/Ser)-NH₂ that protected the myoblasts against menadione-induced cell death. Pre-incubation with the hexapeptide poolreduced the number of cells detached from culture dishsubstrate and increased the ratio of relative viability againstexpression of Bcl-2, an anti-apoptotic protein. These resultssuggest that the hexapeptides might enhance the resistanceto cell death against menadione by increasing the expressionof Bcl-2.

참고문헌 (Reference)

1 Shimada K, "Roles of p38- and c-jun NH2-terminal kinasemediated pathways in 2-methoxyestradiol-induced p53 induction and apoptosis" 24 : 1067-1075, 2003

2 Burlacu A, "Regulation of apoptosis by Bcl-2 family proteins" 7 : 249257-, 2003

3 Niwa K, "Redox regulation of PI3K/Akt and p53 in bovine aortic endothelial cells exposed to hydrogen peroxide" 5 : 713-722, 2003

4 Pinilla C, "Rapid identification of high affinity peptide ligands using positional scanning synthetic peptide combinatorial libraries" 13 : 901-905, 1992

5 Monks TJ, "Quinone chemistry and toxicity. Toxicol Appl Pharmacol" 112 : 2-16, 1992

6 Wagstaff KM, "Protein transduction: celpenetrating peptides and their therapeutic applications" 13 : 1371-1387, 2006

7 Chiou TJ, "Protection of cells from menadioneinduced apoptosis by inhibition of lipid peroxidation" 191 : 77-88, 2003

8 김혜선, "Phosphorylation of Eukaryotic Elongation Factor 2 Can Be Regulated by Phosphoinositide 3-Kinase in the Early Stages of Myoblast Differentiation" 한국분자세포생물학회 21 (21): 294-301, 2006

9 Haddad JJ, "On the antioxidant mechanisms of Bcl-2: a retrospective of NF-kappaB signaling and oxidative stress" 322 : 355-363, 2004

10 Dominov JA, "Muscle-specific BCL2 expression ameliorates muscle disease in laminin {alpha}2-deficient, but not in dystrophin-deficient, mice" 14 : 1029-1040, 2005

11 Rando TA, "Muscle cells from mdx mice have an increased susceptibility to oxidative stress" 8 : 14-21, 1998

12 Chen YR, "Molecular mechanisms of c-Jun N-terminal kinase-mediated apoptosis induced by anticarcinogenic isothiocyanates" 273 : 1769-1775, 1998

13 Houghten RA, "Mixture-based synthetic combinatorial libraries" 42 : 3743-3778, 1999

14 Sun JS, "Menadione-induced cytotoxicity to rat osteoblasts" 53 : 967-976, 1997

15 McArdle F, "Intracellular generation of reactive oxygen species by contracting skeletal muscle cells" 39 : 651-657, 2005

16 Girgenrath M, "Inhibition of apoptosis improves outcome in a model of congenital muscular dystrophy" 114 : 1635-1639, 2004

17 Park JY, "Identification of the peptides that inhibit the stimulation of thyrotropin receptor by Graves’ immunoglobulin G from peptide libraries" 138 : 617-626, 1997

18 Houghten RA, "Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery" 354 : 84-86, 1991

19 Kefaloyianni E, "ERK1/2 and p38- MAPK signalling pathways, through MSK1, are involved in NF-kappaB transactivation during oxidative stress in skeletal myoblasts" 18 : 2238-2251, 2006

20 Rudolf E, "Cytoskeletal changes in nonapoptotic cell death" 49 : 123-128, 2006

21 Basset O, "Bcl-2 overexpression prevents calcium overload and subsequent apoptosis in dystrophic myotubes" 395 : 267-276, 2006

22 Metrailler-Ruchonnet I, "Barazzone and Argiroffo C (2007) Bcl-2 protects against hyperoxia-induced apoptosis through inhibition of the mitochondria-dependent pathway" 42 : 1062-1074, 2007

23 Pinilla C, "Advances in the use of synthetic combinatorial chemistry" 9 : 118-122, 2003