Development of a DNA chip to identify the place of origin of hairtail species

다국어 초록 (Multilingual Abstract)

Hairtails of the family Trichiuridae are widely distributed in the West Sea, South Sea, and Jeju Island in Korea and form large populations on the continental shelf of the western North Pacific. These fish species are imported from China and several other countries because of the high demand in Korea. However,imported hairtail are difficult to distinguish from domestic hairtail. Thus, we developed a DNA chip that distinguishes three hairtail species from eight countries for quick and simple species identification.
Species-specific oligonucleotide probes were designed by sequence analysis of the mitochondrial cytochrome c oxidase subunit I. In this study, we used speciesspecific probes and a DNA chip system to successfully and rapidly identify three different hairtail species from eight different geographical locations.

참고문헌 (Reference)

1 Ivanova, N.V., "Universal primer cocktails for fish DNA barcoding" 7 : 544-548, 2007

2 Lowenstein, J.H., "The real maccoyii: identifying tuna sushi with DNA barcodes-contrasting characteristic attributes and genetic distances" 4 : e7866-, 2009

3 Saitou, N., "The neighbor-joining method: a new method for reconstructing phylogenetic trees" 4 : 406-425, 1987

4 Ward, R., "The campaign to DNA barcode all fishes, FISH-BOL" 74 : 329-356, 2009

5 Yancy, H.F., "Potential use of DNA barcodes in regulatory science: applications of the Regulatory Fish Encyclopedia" 71 : 210-217, 2008

6 Zhang, L., "Position of the fluorescent label is a crucial factor determining signal intensity in microarray hybridizations" 33 : e166-e166, 2005

7 Nijman, V., "Performance of distancebased DNA barcoding in the molecular identification of primates" 333 : 11-16, 2010

8 Tamura, K., "MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0" 24 : 1596-1599, 2007

9 Kochzius, M., "Identifying fishes through DNA barcodes and microarrays" 5 : e12620-, 2010

10 Hubert, N., "Identifying Canadian freshwater fishes through DNA barcodes" 3 : e2490-, 2008

11 Hebert, P.D.N., "Identification of birds through DNA barcodes" 2 : e312-, 2004

12 Chakraborty, A., "Genetic differences among three species of the genus Trichiurus (Perciformes: Trichiuridae) based on mitochondrial DNA analysis" 53 : 93-96, 2006

13 Vrijenhoek, R., "DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates" 3 : 294-299, 1994

14 Kochzius, M., "DNA microarrays for identifying fishes" 10 : 207-217, 2008

15 Rasmussen, R.S., "DNA barcoding of commercially important salmon and trout species (Oncorhynchus and Salmo) from North America" 57 : 8379-8385, 2009

16 Smith, P., "DNA barcoding highlights a cryptic species of grenadier Macrourus in the Southern Ocean" 78 : 355-365, 2011

17 Lakra, W., "DNA barcoding Indian marine fishes" 11 : 60-71, 2011

18 Ward, R.D., "DNA barcoding Australia’s fish species" 360 : 1847-1857, 2005

19 Kress, W.J., "DNA barcodes: genes, genomics, and bioinformatics" 105 : 2761-2762, 2008

20 Tzeng, C.H., "DNA barcode-based identification of commercially caught cutlassfishes (Family: Trichiuridae) with a phylogenetic assessment" 176-181, 2012

21 Peytavi, R., "Correlation between microarray DNA hybridization efficiency and the position of short capture probe on the target nucleic acid" 39 : 89-, 2005

22 Valdez-Moreno, M., "Contreras-Balderas, S. & Hebert, P. Probing diversity in freshwater fishes from Mexico and Guatemala with DNA barcodes" 74 : 377-402, 2009

23 Felsenstein, J., "Confidence limits on phylogenies: an approach using the bootstrap" 783-791, 1985

24 Hebert, P.D.N., "Biological identifications through DNA barcodes" 270 : 313-321, 2003

25 Steinke, D., "Barcoding Nemo: DNA-based identifications for the ornamental fish trade" 4 : e6300-, 2009

26 Kimura, M., "A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences" 16 : 111-120, 1980

27 박정연, "A DNA microarray for species identification of cetacean animals in Korean water" 한국바이오칩학회 4 (4): 197-203, 2010

28 In-Hyuk Chung, "A DNA Microarray for Identification of Selected Korean Birds Based on Mitochondrial Cytochrome c Oxidase I Gene Sequences" 한국분자세포생물학회 30 (30): 295-301, 2010