Intensifying sulforaphane formation in broccoli sprouts by using other cruciferous sprouts additions

다국어 초록 (Multilingual Abstract)

Sulforaphane is a significant chemopreventive compound which is the predominant glucosinolate in broccoli sprouts. However, the existence of the epithiospecifier protein could direct the hydrolysis of glucosinolates toward sulforaphane nitrile formation instead of sulforaphane. Therefore, the study aimed on improving the yielding of sulforaphane in broccoli sprouts with a new method of the united hydrolysis of cruciferous sprouts.
According to the results, the addition of radish, rocket and rape sprouts to broccoli sprouts could promote the hydrolysis of the glucoraphanin to anticancer effective sulforaphane to 2.03, 2.32 and 1.95-fold, respectively, compared to single broccoli sprouts. Meanwhile, the formation of non-bioactive sulforaphane nitrile in these three groups decreased greatly. However, the addition of mustard sprouts had no positive effect. These observations could make a contribution to the potential chemoprotective effects of broccoli sprouts.

참고문헌 (Reference)

1 Sameer Khalil Ghawi, "The potential to intensify sulforaphane formation in cooked broccoli (Brassica oleracea var. italica) using mustard seeds (Sinapis alba)" Elsevier BV 138 (138): 1734-1741, 2013

2 Judson, H.G., "The incidence of goitre in newborn lambs from ewes fed fodder radish, rape or Italian ryegrass with or without iodine supplementation" 71 : 66-70, 2011

3 Hao Liang, "Sulforaphane Induces Cell-Cycle Arrest and Apoptosis in Cultured Human Lung Adenocarcinoma LTEP-A2 Cells and Retards Growth of LTEP-A2 Xenografts in Vivo" American Chemical Society (ACS) 71 (71): 1911-1914, 2008

4 Zahra Bahadoran, "Potential Efficacy of Broccoli Sprouts as a Unique Supplement for Management of Type 2 Diabetes and Its Complications" 한국식품영양과학회 16 (16): 375-382, 2013

5 Hoffmann, "Modulation of drug metabolizing enzymes by dietary doses of sulforaphane; role in its anti-hypertensive and anti-oxidant effect in spontaneously hypertensive rats" 6 : 2016

6 Cristina Angeloni, "Modulation of Phase II Enzymes by Sulforaphane: Implications for Its Cardioprotective Potential" American Chemical Society (ACS) 57 (57): 5615-5622, 2009

7 Dosz, E.B., "Modifying the processing and handling of frozen broccoli for increased sulforaphane formation" 58 : 1459-1463, 2013

8 Franziska S. Hanschen, "Isothiocyanates, Nitriles, and Epithionitriles from Glucosinolates Are Affected by Genotype and Developmental Stage in Brassica oleracea Varieties" Frontiers Media SA 8 : 2017

9 Dan Song, "Instability and Structural Change of 4-Methylsulfinyl-3-butenyl Isothiocyanate in the Hydrolytic Process" American Chemical Society (ACS) 61 (61): 5097-5102, 2013

10 Grace C. Wang, "Impact of Thermal Processing on Sulforaphane Yield from Broccoli (Brassica oleracea L. ssp. italica )" American Chemical Society (ACS) 60 (60): 6743-6748, 2012

11 Doris Lippmann, "Glucosinolates from pak choi and broccoli induce enzymes and inhibit inflammation and colon cancer differently" Royal Society of Chemistry (RSC) 5 (5): 1073-1081, 2014

12 Steven F. Vaughn, "Glucosinolate hydrolysis products from various plant sources: pH effects, isolation, and purification" Elsevier BV 21 (21): 193-202, 2005

13 Ruimin Li, "Glucoraphenin, sulforaphene, and antiproliferative capacity of radish sprouts in germinating and thermal processes" Springer Nature 243 (243): 547-554, 2017

14 Guo, L, "Glucoraphanin, sulforaphane and myrosinase activity in germinating broccoli sprouts as affected by growth temperature and plant organs" 5 : 70-77, 2014

15 Nathan V. Matusheski, "Epithiospecifier Protein from Broccoli ( Brassica oleracea L. ssp. italica ) Inhibits Formation of the Anticancer Agent Sulforaphane" American Chemical Society (ACS) 54 (54): 2069-2076, 2006

16 Yuesheng ZHANG, "Discovery and development of sulforaphane as a cancer chemopreventive phytochemical" Springer Nature 28 (28): 1343-1354, 2007

17 Nathan V. Matusheski, "Comparison of the Bioactivity of Two Glucoraphanin Hydrolysis Products Found in Broccoli, Sulforaphane and Sulforaphane Nitrile" American Chemical Society (ACS) 49 (49): 5743-5749, 2001

18 Ambrosone, C.B, "Breast cancer risk in premenopausal women is inversely associated with consumption of Broccoli, a source of isothiocyanates, but is not modified by GST genotype" 134 : 1134-1138, 2004

19 John D. Clarke, "Bioavailability and inter-conversion of sulforaphane and erucin in human subjects consuming broccoli sprouts or broccoli supplement in a cross-over study design" Elsevier BV 64 (64): 456-463, 2011

20 Kristina Radošević, "Assessment of glucosinolates, antioxidative and antiproliferative activity of broccoli and collard extracts" Elsevier BV 61 : 59-66, 2017

21 Nan Wang, "Analysis of the isothiocyanates present in three Chinese Brassica vegetable seeds and their potential anticancer bioactivities" Springer Nature 231 (231): 951-958, 2010