Destruction of Tissue Architecture Induced by Dust Particles in Inkjet Bioprinted Alveolar Barrier

다국어 초록 (Multilingual Abstract)

Atmosphere dust particles travel into our body via the airway and cause damages to our respiratory system, which causes severe respiratory diseases. Therefore, the need for studies to analyze the effects of dust particles on the respiratory system has been emphasized. However, most studies about the toxicity of dust have been carried out in two-dimensional cell culture, animal models, and epidemiological investigations. To figure out how dust can cause respiratory disease, it is necessary to examine using a reliable three-dimensional structured model, which mimics human nature alveoli. In this study, we applied atmospheric dust particles in dose- and time-dependent manner on our previously developed three-dimensional alveolar barrier, which is generated by the inkjet bioprinting process. As results, we observed destruction of tissue architecture along with cell death in our engineered alveolar barrier. Based on the damages in cellular levels, we observed increased pro-inflammatory cytokines, which trigger the signal transduction pathway leading to the activation of transcription factors. As cascades of release of cytokines, we confirmed a degradation of extracellular matrix, which might induce a collapse of the structure, loss of cell polarity, and a decreased barrier tightness. We further investigated pulmonary surfactant protein-related genes in dust-treated alveolar tissue then we could estimate the possible harmful effect of dust on pulmonary surfactant dysfunction. This study demonstrated the physiological impact of dust on cytotoxicity effects, alveolar barrier rigidity, and surfactant secretion using inkjet bioprinted alveolar barrier in gene expression level. Additionally, it has been demonstrated that dust can have serious consequences that can lead to the collapse of the tissue structure. We expect that this strategy using in vitro inkjet bioprinted 3D alveolar barrier can be a useful tool for identifying pollutant exposure-related diseases.