크리신(chrysin)이 리포테이코산과 poly-IC로 자극된 마우스 대식세포 RAW 264.7의 hydrogen peroxide 생성에 미치는 영향 = Effects of chrysin on hydrogen peroxide productions in RAW 264.7 mouse macrophages stimulated by lipoteichoic acid and poly-IC

다국어 초록 (Multilingual Abstract)

Objectives : This study aimed to elucidate antioxidant activity of chrysin in polyinosinic-polycytidylic acid (poly-IC) and lipoteichoic acid-induced RAW 264.7 mouse macrophages.
Methods : RAW 264.7 co-stimulated with poly-IC and lipoteichoic acid were incubated with chrysin at concentrations of 25 and 50 μM. Hydrogen peroxide production was measured with dihydrorhodamine 123 assay. Nitric Oxide (NO) production was evaluated by griess reagent assay.
Results : For 16 h, 18 h, 20 h, 22 h, and 24 h incubation, chrysin at the concentration of 25 and 50 μM significantly suppressed hydrogen peroxide production in poly-IC and lipoteichoic acid-induced RAW 264.7. In details, production of hydrogen peroxide in ‘poly-IC and lipoteichoic acid’-stimulated RAW 264.7 treated for 16 h with chrysin at concentrations of 25 and 50 μM was 83.84% and 79.3% of the control group treated with poly-IC and lipoteichoic acid only, respectively; the production of hydrogen peroxide for 18 h was 84.36% and 79.93%, respectively; production of hydrogen peroxide for 20 h was 85.68% and 80.22%, respectively; production of hydrogen peroxide for 22 h was 85.81% and 79.95%, respectively; production of hydrogen peroxide for 24 h was 86.01% and 80.18%, respectively. Additionally, chrysin at the concentration of 5, 10, 25, and 50 μM significantly inhibited NO production in THP-1 human monocytic cell line.
Conclusions : Chrysin might have anti-oxidative activity related to its inhibition of hydrogen peroxide production in ‘poly-IC and lipoteichoic acid’-stimulated RAW 264.7.