Anthocyanin Accumulation and Expression of Anthocyanin Biosynthetic Genes in Radish (<i>Raphanus sativus</i>)

다국어 초록 (Multilingual Abstract)

<P>Radish [<I>Raphanus sativus</I> (Rs)] is an important dietary vegetable in Asian countries, especially China, Japan, and Korea. To elucidate the molecular mechanisms of anthocyanin accumulation in radish, the gene expression of enzymes directly involved in anthocyanin biosynthesis was analyzed. These genes include phenylalanine ammonia lyase (<I>PAL</I>), cinnamate 4-hydroxylase (<I>C4H</I>), 4-coumarate–CoA ligase (<I>4CL</I>), chalcone synthase (<I>CHS</I>), chalcone isomerase (<I>CHI</I>), flavanone 3-hydroxylase (<I>F3H</I>), dihydroflavonol reductase (<I>DFR</I>), and anthocyanidin synthase (<I>ANS</I>). <I>RsDFR</I> and <I>RsANS</I> were found to accumulate in the flesh or skin of two radish cultivars (Man Tang Hong and Hong Feng No.1). Radish skin contained higher <I>CHS</I>, <I>CHI</I>, and <I>F3H</I> transcript levels than radish flesh in all three cultivars. In the red radish, 16 anthocyanins were separated and identified by high-performance liquid chromatography (HPLC) and elctrospray ionization–tandem mass spectrometry (ESI–MS/MS). Some of them were acylated with coumaroyl, malonoyl, feruoyl, and caffeoyl moieties. Furthermore (−)-epicatechin and ferulic acid were also identified in the three cultivars.</P><P><B>Graphic Abstract</B>
<IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2011/jafcau.2011.59.issue-11/jf200824c/production/images/medium/jf-2011-00824c_0002.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf200824c'>ACS Electronic Supporting Info</A></P>