보리와 밀 맥아 Amylases 의 반응속도론적 연구 = Kinetic Studies on Amylases from Barley and Wheat Malt

다국어 초록 (Multilingual Abstract)

α-amylase and β-amylase were extracted from barley and wheat malt, respectively. Their kinetic parameters on gultinous and nonglutinous rice starch were examined. During the germination of barley and wheat, the increaments of ATP levels were significant after 2-day germination and the levels were reduced after 5 days. The dry weights were decreased after 3 days. The activities of amylases were the highest for 6 days in the barley and wheat malt. As for α-amylase, that the substrate affinity of barley malt on nonglutionous rice starch was greater than other cases. The V_(max) values of α-amylase from wheat malt on either type of rice starch showed high, and from barley malt on nonglutinous rice starch were high. The β-amylse from barley malt showed high substrate affinity on the glutinous rice starch, and V_(max) value of the enzyme from wheat malt on glutinous rice starch was higher than other. The substrate efficiency (V_(max)/K_m) of β-amylase on the nonglutinous rice strach was better than other cases.