편백의 다신초 유도 및 발근을 통한 식물체 재분화 = In vitro regeneration of Chamaecyparis obtusa

다국어 초록 (Multilingual Abstract)

A protocol for the in vitro propagation of Chamaecyparis obtusa was established in the present study. Multi-shoots were initiated from apical shoot explants from germinants after 10 weeks of culture on Litvay medium (LM) supplemented with different concentrations of cytokinin. The effects of pre-treatment with high concentrations of cytokinin and varying concentrations (0.2 to 5.0 mg/L) of zeatin on in vitro shoot elongation and shoot multiplication were investigated. Optimal shoot growth was achieved on LM medium, with over 10-mm shoots after 10 weeks of culture. In the anti-browning tests, ethanesulfonic acid triggered the least browning in the shoot tips. The highest multi-shoot induction was observed in the 0.5-mg/L zeatin treatments, which yielded 80% induction of shoots after 10 weeks of culture, and maximum shoot elongation was observed in the LM basal medium without the hormone. The highest rooting rates were 65% under 0.2 mg/L indole-3- butyric acid.

참고문헌 (Reference)

1 김정희, "편백나무 잎 추출물의 성분분석과 면역효능에 관한 연구" 대한임상검사과학회 50 (50): 37-43, 2018

2 Benson EE, "The use of the iron chelating agent desferrioxamine in rice cell cryopreservation: a novel approach for improving recovery" 110 : 249-258, 1995

3 Arnold SV, "The influence of cytokinin pulse treatments on adventitious bud formation on vegetative buds of Picea abies" 9 : 253-261, 1987

4 Taniguchi T, "Somatic embryogenesis and plant regeneration from immature zygotic embryos of Hinoki cypress (Chamaecyparis obtusa Sieb. et Zucc.)" 23 : 26-31, 2004

5 Maruyama E, "Somatic embryo culture for propagation, artificial seed production, and conservation of sawaracypress (Chamaecyparis pisifera Sieb. et Zucc.)" 8 : 1-8, 2003

6 Okamura M, "Rooting from the encapsulated adventitious buds of Hinoki, Chamaecyparis obtusa" 76 : 601-603, 1994

7 Malabadi RB, "Role of antioxidants and amino acids on somatic embryogenesis of Pinus patula" 41 : 181-186, 2005

8 Ishii K, "Plant regeneration by somatic embryogenesis from in vitro-cultured shoots of hinoki cypress(Chamaecyparis obtusa Sieb. et Zucc.)" 3 : 19-22, 2003

9 Teixeira da Silva JA, "Phloroglucinol in plant tissue culture" 49 : 1-16, 2013

10 Teasdale RD, "Mineral nutrient requirements of a loblolly pine (Pinus taeda) cell suspension culture" 82 : 942-945, 1986

11 Kagenishi T, "MES buffer affects Arabidopsis root apex zonation and root growth by suppressing superoxide generation in root apex" 7 : 79-, 2016

12 Litvay JD, "Influence of loblolly pine (Pinus taeda L.) culture medium and its components on growth and somatic embryogenesis of the wild carrot (Daucus carota L.)" 4 : 325-328, 1985

13 Katy A. Nour, "In vitro shoot multiplication of eastern white cedar (Thuja occidentals)" 29 : 65-71, 1993

14 Min JY, "In vitro propagation of Chamaecyparis obtusa sieb. et Zucc" 10 : 117-121, 2010

15 Ishii K, "In vitro plantlet formation from adventitious buds on juvenile seedlings of Hinoki cypress (Chamaecyparis obtusa)" 7 : 247-255, 1986

16 Benson EE, "Do free radicals have a role in plant tissue culture recalcitrance?" 36 : 163-170, 2000

17 Joseph J. Kieber, "Cytokinin signaling in plant development" The Company of Biologists 145 (145): dev149344-, 2018

18 Lee SW, "Containerized seedling production system of Chamaecyparis obtusa" 413 : 30-, 2011

19 Sasamoto H, "Callus regeneration from cotyledon protoplasts of Chamaecyparis obtusa (Hinoki cypress)" 28 (28): 132-136, 1992

20 Khamushi M, "An efficient protocol for micropropagation of old cypress of Abarkuh (Cupressus sempervirens var. horizontalis [Mill.]) under in vitro condition" 138 : 597-601, 2019

21 Forest Resource Division, Forest Industry, "2019 Forest Resource Section Business Plan" 7-, 2019