桑심子추출물의 Peroxynitrite 억제 및 염증 촉진 인자 제어 효과 = The inhibitory effects of Mori Fructus on the reactive species and proinflammatory proteins

다국어 초록 (Multilingual Abstract)

Objectives : This study was to investigate the inhibitory effects of Mori Fructus on the generation of peroxynitrite (ONOO-), nitric oxide (NO) and superoxide anion radical (?O2-) in the endothelial cells of rat vessels. The effects of Mori Fructus on the expression of inflammation-related proteins, NF-κB (p50, p65), COX-2, and iNOS, were examined by western blotting.
Methods : For this study, the fluorescent probes, namely dihydrorhodamine 123 (DHR 123), 4,5-diaminofluorescein (DAF-2) and 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) were used. Western blotting was performed using anti-NF-κB (p50, p65), anti-COX-2, anti-iNOS respectively.
Results : Mori Fructus inhibited the generation of ONOO-, NO and ?O2- in the lipopolysaccharide (LPS)-treated endothelial cells of rat vessels in vitro. Mori Fructus inhibited the expression of COX-2 and iNOS genes by means of decreasing the NF-κB activation.
Conclusions : hese results suggest Mori Fructus is effective on inhibiting the generation of ONOO-, NO and ?O2-, and that therefore it might have a potential role as a treatment for the inflammatory process and inflammation-related diseases.

목차 (Table of Contents)

• I. 緖論 = 1
• II. 材料 및 方法 = 3
• 1. 재료 = 3
• 1) 약재 = 3
• 2) 시약 및 기기 = 3
• 2. 방법 = 3
• 1) 검액의 조제 = 3
• 2) 세포 배양 = 4
• 3) MTT assay = 4
• 4) 혈관내피세포에서 ONOO^(-), NO 및 ·O₂^(-) 소거능 측정 = 5
• 5) Western blot analysis = 5
• 6) 단백질 정량 = 6
• 7) 통계 처리 = 6
• III. 成績 = 7
• 1. 상심자의 세포 독성 측정 = 7
• 2. 상심자의 세포 보호 효과 = 7
• 3. LPS 처리 혈관내피세포에서 ONOO^(-), NO 및 ·O₂^(-) 생성에 미치는 영향 = 7
• 4. LPS 처리 혈관내피세포에서 핵내의 NF-κB 단백질 양에 미치는 영향 = 8
• 5. LPS 처리 혈관내피세포에서 염증 촉진 인자의 발현에 미치는 영향 = 8
• IV. 考察 = 18
• V. 結論 = 21
• 參考文獻 = 22
• ABSTRACT = 26