생쥐 시각피질에서 melanopsin을 가지는 신경세포의 면역조직화학적 위치

국문 초록 (Abstract)

Melanopsin은 옵신과 비슷한 광색소로 망막 광민감성 신경절 세포에서 적은 비율로 발견 된다. Melanopsin은 일주기 리듬조절에 관여 하고 동공반사를 조절한다. 이번 연구의 목적은 melanopsin이 망막 이외의 중추 시각계 에서도 발견 되는지를 알아 보는 것이다. 우리는 생쥐 시각 피질에 존재하는 melanopsin을 가지는 신경세포를 면역조직화학법을 통해 염색하여 melanopsin의 분포와 형태를 분석하였다. Melanopsin 면역반응은 핵을 제외한 신경세포의 세포체에서 일어났다. 우리는 melanopsin이 성체 생쥐의 시각 피질의 2,3층에서 높은 밀도로 모여서 존재하는 것을 확인했다. 시각 피질 1층에서 melanopsin 면역반응을 보인 신경세포는 드물게 발견 되었다. 생쥐 시각 피질에서 발견된 melanopsin의 세포종류는 주로 round/oval 세포였으나, vertical fusiform 그리고 pyramidal 세포 등도 드물게 발견 되었다. 염색된 세포들의 분포는 특이적이지 않았다. 우리의 실험 데이터로 melanopsin이 시각피질에도 분포한다는 것을 알 수 있었다. 또한, melanopsin을 가지는 신경세포는 nitric oxide synthase, calbindin and parvalbumin과도 같이 발현 되는 것을 관찰했다. 이러한 실험결과는 망막 이외의 부분에서 발견되는 melanopsin의 기능에 대한 연구가 필요함을 야기한다.

다국어 초록 (Multilingual Abstract)

Melanopsin is an opsin-like photopigment found in the small proportion of photosensitive ganglion cells of the retina. It is involved in the regulation of the synchronization of the circadian cycle as well as in the control of pupillary light reflex. The purpose of the present study is to investigate whether melanopsin is also expressed in the other areas of the central visual system outside the retina. We have studied the distribution and morphology of neurons containing melanopsin in the mouse visual cortex with antibody immunocytochemistry. Melanopsin immunoreactivity was mostly present in neuronal soma, but not in nuclei. We found that melanopsin was present in a large subset of neurons within the adult mouse visual cortex with the highest density in layer II/III. In layer I of the visual cortex, melanopsin-immunoreactive (IR) neurons were rarely encountered. In the mouse visual cortex, the majority of the melanopsin-IR neurons consisted of round/oval cells, but was varied in morphology. Vertical fusiform and pyramidal cells were also rarely labeled with the anti-melanopsin antibody. The labeled cells did not show any distinctive distributional pattern. Some melanopsin-IR neurons in mouse visual cortex co-localized with nitricoxide synthase, calbindin and parvalbumin. Our data indicate that melanopsin is located in specific neurons and surprisingly widespread in visual cortex. This finding raises the need of the functional study of melanopsin in central visual areas outside the retina.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 결과
• 고찰
• References
• 초록

참고문헌 (Reference)

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