Detection of Oyster-Associated Norovirus by Microchip Electrophoresis of an Amplified cDNA

다국어 초록 (Multilingual Abstract)

Noroviruses, members of the family Caliciviridae, are often found in shellfish grown in polluted water and are emerging as a leading cause of foodborne disease worldwide. As the presence of norovirus in food commodities becomes an important medical and social issue, there are increasing needs for designing improved detection methods for the virus. In this study, we tested the Agilent 2100 Bioanalyzer for the analysis of norovirus DNA amplified from oyster samples. Microchip electrophoresis provided us with more accurate information, compared to conventional agarose gel electrophoresis, in the resolution and quantification of amplified products. The development of an improved method for food-associated noroviruses would contribute to a rapid identification of contaminated food and improve our understanding of the modes of food contamination and norovirus transmission.

참고문헌 (Reference)

1 "Viruses and bivalve shellfish" 59 : 81-116, 2000

2 "Three-year study to assess human enteric viruses in shellfish" 66 : 3241-3248, 2000

3 "Reverse transcriptase PCR detection of astrovirus, hepatitis A virus, and polio-virus in experimentally contaminated mussels: comparison of several extraction and concentration methods" 64 : 3118-3122, 1998

4 "Rapid and sensitive detection of noroviruses by using TaqMan-based one-step reverse transcription-PCR assays and application to naturally contaminated shellfish samples" 71 : 1870-1875, 2005

5 "Multiplex nucleic acid sequence-based amplification for simultaneous detection of several enteric viruses in model ready-to-eat foods" 70 : 6603-6610, 2004

6 "International collaborative study to compare reverse transcriptase PCR assays for detection and genotyping of noroviruses" 41 : 1423-1433, 2003

7 "Genogroup-specific PCR primers for detection of Norwalk-like viruses" 100 : 107-114, 2002

8 "Genetic classification of "Norwalk-like viruses" 181 : S336-S348, 2000

9 "Foodborne viruses: an emerging problem" 90 : 23-41, 2004

10 "Foodborne viruses" 26 : 187-205, 2002

11 "Diagnosis of noncultivatable gastroenteritis viruses, the human caliciviruses" 14 : 15-37, 2001

12 "Detection, quantitation, and phylogenetic analysis of noroviruses in Japanese oysters" 69 : 5782-5786, 2003

13 "Detection of small round structured viruses in shellfish by reverse transcription-PCR" 61 : 4418-4424, 1995

14 "Detection of both hepatitis A virus and Norwalk-like virus in imported clams associated with food-borne illness" 68 : 3914-3918, 2002

15 "Detection of DNA mutations associated with mitochondrial diseases by Agilent 2100 bioanalyzer" 318 : 97-105, 2002

16 "Coexistence of multiple genotypes, including newly identified genotypes, in outbreaks of gastroenteritis due to norovirus in Japan" 42 : 2988-2995, 2004

17 "Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription- PCR" 41 : 1548-1557, 2003