인체 치은섬유모세포에 대한 니켈의 세포독성과 Ferulic Acid 및 Vitamin E의 독성경감효과 = The Inhibitory Effects of Ferulic Acid and Vitamin E on Nickel-Induced Cytotoxicity in Human Gingival Fibroblasts

다국어 초록 (Multilingual Abstract)

Nickel is major metal used in the nickel-chromium alloys of most orthodontic appliances and partial denture. But this metal is known to cause hypersensitivity, dermatitis and asthma. In addition, significant carcinogenic and mutagenic potentials has been demonstrated for compounds containing nickel. The purpose of this study was to develop the compounds repairing on the cytotoxicity of nickel in cultured human gingival fibroblasts. The cytotoxicity of nickel sulfide and nickel oxide in human gingival fibrobalsts treated with dose dependent nickel was measured by MTT assay for cell viability and XTT assay for cell adhesion activity. The cytotoxicity of ferulic acid itself also was investigated by the same cells and methods. The procedure of repairment in human gingival fibroblasts injuried by NiS_50 (nickel sulfide IC_50) and Ni0_50 (nickel oxide IC_50) was carried out to observe after treatment of ferulic acid by dose dependent manner. The 5×10⁴ cells/㎖ of human gingival fibroblasts in each well of 24 multidish were cultured. After 24 hrs, the cells were treated with solution of all groups. After the human gingival fibroblasts were cultured in same condition for 48 hrs, the colormetric methods were performed to evaluate the cytotoxicity. The light microscopic study was carried out to observe morphological changes of cultured human gingival fibroblasts.
The results were as follows :
1. According to the MTT absorbance, nickel sulfide and nickel oxide were significantly decreased in 25μM and 50μM, respectively. IC_50 of nickel sulfide and nickel oxide were 138.1μM and 311.7μM, respectively.
2. According to the XlT absorbance, nickel sulfide was significantly decreased in 50 μM and nickel oxide was significantly decreased in 100μM, IC_50 of nickel sulfide and nickel oxide were 104.4μlM and 367.8μM, respectively.
3. MTT_50 and XTT_50 of ferulic acid were 2,130.3μM and 1,773.7μM, respectively. These results were determind to nontoxic by Borenfreund at., al.
4. The inhibitory effects of ferulic acid against nickel-induced cytotoxicity were significantly increased from 25μM ferulic acid, but inhibitory effects of 100μM ferulic acid was more decreased than that of 50μM ferulic acid.
5. Treatment of ferulic acid and vitamin E combination was more assumed an upward curve than that of ferulic acid only.
6. The cell numbers and cell shapes were repaired from 1μM ferulic acid. However 100μM ferulic acid showed degeneration again.
These results suggest that ferulic acid and vitamin E may be decrease the cytotoxicity of nickel sulfide and nickel oxide in cultured human gingival fibroblasts.

목차 (Table of Contents)

• Ⅰ. 서론
• Ⅱ. 재료 및 방법
• Ⅲ. 연구 성적
• Ⅳ. 총괄 및 고찰
• Ⅴ. 결론
• 참고문헌
• Explanation of Photos
• ABSTRACT