Inositol 5'-phosphatase, SHIP1 interacts with phospholipase C-γ1 and modulates EGF-induced PLC activity

다국어 초록 (Multilingual Abstract)

Phospholipase C-γ1, containing two SH2 and one SH3 domains which participate in the interaction between signaling molecules, plays a significant role in the growth factor-induced signal transduction. However, the role of the SH domains in the growth factor-induced PLC-γ1 regulation is unclear. By peptide-mass fingerprinting analysis, we
have identified SHIP1 as the binding protein for the SH3 domain of PLC-γ1. SHIP1 was co-immunoprecipitated with PLC-γ1 and potentiated EGF-induced PLC-γ1 activation. However, inositol 5'-phosphatase activity of SHIP1 was not required for the potentiation of EGF-induced PLC-γ1 activation. Taken together, these results suggest that SHIP1 may function as an adaptor protein which can potentiate EGF-induced PLC-γ1 activation without regards to its inositol 5'-phosphatase activity.

참고문헌 (Reference)

1 "throughmembrane targeting by Ras" 2752-7,

2 "and a 150-kilodalton tyrosinephosphorylatedprotein form complexes with Fms in hematopoieticcells" 5682-91,

3 "The mechanism of phospholipaseC-gamma1 regulation" 101-9, expmolmed2000;32

4 "The RasGAP-binding protein p62dokis a mediator of inhibitory FcgammaRIIB signals in B cells" 347-58, immunity2000;12

5 "The 145-kDa protein induced toassociate with Shc by multiple cytokines is an inositol tetraphosphateand phosphatidylinositol 3" 5 1689-93,

6 "Src homology 2-containing inositol5-phosphatase 1 binds to the multifunctional docking site ofc-Met and potentiates hepatocyte growth factor-inducedbranching tubulogenesis" 3017-23,

7 "Sequence similarity of phospholipase C with the non-catalyticregion of src" 269-72, nature1988;332

8 "SIP/SHIP inhibits Xenopus oocyte maturationinduced by insulin and phosphatidylinositol 3-kinase" 2559-65, molcellbiol1997;17

9 "SH3 domains direct cellular localization of signaling molecules" 83-91,

10 "Regulation of phosphoinositide-specificphospholipase C isozymes" 15045-8,

11 "Recruitment and phosphorylation ofSH2-containing inositol phosphatase and Shc to the B-cellFc gamma immunoreceptor tyrosine-based inhibition motifpeptide motif" 4305-11,

12 "Phosphorylationof glycogen synthase kinase-3beta at serine-9 byphospholipase Cgamma1 through protein kinase C in rat 3Y1fibroblasts" 444-50, expmolmed2002;34

13 "PLC gamma 1 Src homology domaininduces mitogenesis in quiescent NIH 3T3 fibroblasts" 222 : 186-93

14 "Overexpression of phospholipase C-gamma1 in rat3Y1 fibroblast cells leads to malignant transformation" 5465-8, cancerres1997;57

15 "Identification of a second SH2-domain-containing proteinclosely related to the phosphatidylinositol polyphosphate 5-phosphatase SHIP" 697-700,

16 "Human type II Fcgammareceptors inhibit B cell activation by interacting with thep21" 30499-504,

17 "Erythropoietin stimulatesthe tyrosine phosphorylation of Shc and its associationwith Grb2 and a 145-Kd tyrosine phosphorylatedprotein" 2296-303,

18 "Detection of Src homology 3-binding proteins" includingpaxillin 14956-63,

19 "Deletion of SHIP or SHP-1 reveals two distinct pathwaysfor inhibitory signaling Phospholipase C-gamma 1binds to actin-cytoskeleton via its C-terminal SH2 domain invitro" 293 802-6,

20 "Cross-linking of IgG receptors inhibits membraneimmunoglobulin-stimulated calcium influx in B lymphocytes" 355-63,

21 "Cloningand sequence of multiple forms of phospholipase C" 161-9, cell1988;54

22 "An alternative to SH2 domainsfor binding tyrosine-phosphorylated proteins" science1994;2661862-5

23 "A novel spliced form of SH2-containing inositol phosphatase is expressed during myeloiddevelopment" 1922-33