폐경 쥐 모델에서 두류 섭취에 따른 자궁의 비타민 D 수용체의 발현 = Expression of Vitamin D Receptor by Pulse Consumption in the Uterus of Menopausal Mouse Model

다국어 초록 (Multilingual Abstract)

Objectives: Phytoestrogen-containing pulse supplements have beneficial effects on postmenopausal symptoms, but how such effects are achieved is unclear. This study investigates the effects of pulse consumption on the menopausal changes in ovariectomized rats. Methods: Female Sprague-Dawley rats were either sham operated (Sham; n = 3) or surgically ovariectomized (n = 13). The Sham group was fed the regular AIN-93M diet. Ovariectomized group was divided into 3 sub-groups and fed AIN-93M containing soybean (n = 5), mung bean (n = 3), or cowpea (n = 5) for 10 weeks. At the end of the experiment, all rats were sacrificed, and the uterus was harvested, rinsed, and weighed. Expressions of vitamin D receptor (VDR), estrogen receptor (ER) β, and ezrin in uterus were evaluated by immunohistochemistry. Results: VDR was highly expressed in the uterus of rat, irrespective of ovariectomized state. VDR was more definitely expressed in the uterus of ovariectomized groups than the sham-operated group. There were no significant differences in expression of ER β. However the expression of ezrin was highly expressed in the cowpea group compared to sham group (P = 0.044). Conclusion: This study suggested that legumes diet may concern menopausal changes via VDR and ezrin. The result may partly explain the beneficial effects of VDR on menopausal symptoms. Further study is necessary to study the detailed mechanisms of VDR and ezrin on the menopausal changes in the uterus. (J Korean Soc Menopause 2013;19:1-8)

참고문헌 (Reference)

1 Zarnani AH, "Vitamin D3 receptor is expressed in the endometrium of cycling mice throughout the estrous cycle" 93 : 2738-2743, 2010

2 Lips P, "Vitamin D physiology" 92 : 4-8, 2006

3 Smith PM, "Tissuespecific regulation by estrogen of ezrin and ezrin/radixin/ moesin-binding protein 50" 22 : 119-126, 2003

4 Kuiper GG, "The novel estrogen receptorbeta subtype: potential role in the cell- and promoterspecific actions of estrogens and anti-estrogens" 410 : 87-90, 1997

5 Khanna C, "The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis" 10 : 182-186, 2004

6 Yildirim B, "The effects of postmenopausal vitamin D treatment on vaginal atrophy" 49 : 334-337, 2004

7 Adams MR, "The atheroprotective effect of dietary soy isoflavones in apolipoprotein E-/- mice requires the presence of estrogen receptor-alpha" 22 : 1859-1864, 2002

8 Lee SK, "Study on the impact of isoflavone in hormonal profiles during the menstrual cycle and in the postmenopausal parameters in Korean women" Yonsei University 2001

9 Chen YM, "Soy isoflavones have a favorable effect on bone loss in Chinese postmenopausal women with lower bone mass: a doubleblind, randomized, controlled trial" 88 : 4740-4747, 2003

10 Harkness L, "Soy and bone. Where do we stand" 23 : 12-17, 2004

11 Roach VJ, "Phytoestrogens: dietary intake and excretion in postmenopausal Chinese women" 1 : 290-295, 1998

12 Lu LJ, "Phytoestrogens and healthy aging: gaps in knowledge. A workshop report" 8 : 157-170, 2001

13 Baeza I, "Ovariectomy, a model of menopause in rodents, causes a premature aging of the nervous and immune systems" 219 : 90-99, 2010

14 Messina MJ, "Legumes and soybeans: overview of their nutritional profiles and health effects" 70 : 439S-450S, 1999

15 Alekel DL, "Isoflavone-rich soy protein isolate attenuates bone loss in the lumbar spine of perimenopausal women" 72 : 844-852, 2000

16 Enmark E, "Human estrogen receptor beta-gene structure, chromosomal localization, and expression pattern" 82 : 4258-4265, 1997

17 Holick MF, "High prevalence of vitamin D inadequacy and implications for health" 81 : 353-373, 2006

18 Curto M, "Ezrin...a metastatic detERMinant" 5 : 113-114, 2004

19 Hunter KW, "Ezrin, a key component in tumor metastasis" 10 : 201-204, 2004

20 Hiscox S, "Ezrin regulates cell-cell and cellmatrix adhesion, a possible role with E-cadherin/betacatenin" 112 Pt 18 : 3081-3090, 1999

21 Heldring N, "Estrogen receptors: how do they signal and what are their targets" 87 : 905-931, 2007

22 Cavallini A, "Estrogen receptor (ER) and ER-related receptor expression in normal and atrophic human vagina" 59 : 219-225, 2008

23 Hess RA, "Estrogen in the adult male reproductive tract: a review" 1 : 52-, 2003

24 Nashold FE, "Estrogen controls vitamin D3-mediated resistance to experimental autoimmune encephalomyelitis by controlling vitamin D3 metabolism and receptor expression" 183 : 3672-3681, 2009

25 Song J, "Estradiol-induced ezrin overexpression in ovarian cancer: a new signaling domain for estrogen" 220 : 57-65, 2005

26 Chiechi LM, "Efficacy of a soy rich diet in preventing postmenopausal osteoporosis: the Menfis randomized trial" 42 : 295-300, 2002

27 Knight DC, "Effects on menopausal symptoms and acceptability of isoflavonecontaining soy powder dietary supplementation" 4 : 13-18, 2001

28 Marini H, "Effects of the phytoestrogen genistein on bone metabolism in osteopenic postmenopausal women: a randomized trial" 146 : 839-847, 2007

29 Mosselman S, "ER beta: identification and characterization of a novel human estrogen receptor" 392 : 49-53, 1996

30 Adlercreutz H, "Dietary phyto-oestrogens and the menopause in Japan" 339 : 1233-, 1992

31 Athanasopoulou A, "Decreased ezrin and paxillin expression in human urothelial bladder tumors correlate with tumor progression" 2011

32 Tremblay GB, "Cloning, chromosomal localization, and functional analysis of the murine estrogen receptor beta" 11 : 353-365, 1997

33 Anderson JW, "Cardiovascular and renal benefits of dry bean and soybean intake" 70 : 464S-474S, 1999

34 McDonnell DP, "Capitalizing on the complexities of estrogen receptor pharmacology in the quest for the perfect SERM" 949 : 16-35, 2001

35 Lichtenstein AH, "Approaches to reducing cardiovascular disease risk: food or pills" 12 : 1-3, 2001

36 Song AR, "A study on management of menopause of climacteric women" 3 : 140-160, 1997

37 Somjen D, "A non-calcemic vitamin D analog modulates both nuclear and putative membranal estrogen receptors in cultured human vascular smooth muscle cells" 89-90 : 397-399, 2004

38 Francis RM, "A comparison of the effects of alfacalcidol treatment and vitamin D2 supplementation on calcium absorption in elderly women with vertebral fractures" 6 : 284-290, 1996

39 Pan Y, "A comparison of oral micronized estradiol with soy phytoestrogen effects on tail skin temperatures of ovariectomized rats" 8 : 171-174, 2001