DNA capturing machinery through spore‐displayed proteins

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P><P><B>Aims: </B> The purpose of this study was to develop a general method for the facile development of a new DNA biosensor which utilizes streptavidin‐displayed spores as a molecular machinery.</P><P><B>Methods and Results: </B> Fluorescence spectroscopy was used as a monitoring tool for the streptavidin displayed on the surface of <I>Bacillus thuringiensis</I> spores and as a diagnosis method for DNA detection. As a proof‐of‐concept, four pathogenic bacteria including <I>Pseudomonas aeruginosa</I>, <I>Acinetobacter baumannii</I>, <I>Escherichia coli</I> and <I>Klebsiella pneumonia</I> were used for the detection of pathogenic species. In addition, a set of mutant variants of Wilson’s disease were also used for the detection of single nucleotide polymorphism (SNP) in this system.</P><P><B>Conclusions: </B> This strategy, utilizing streptavidin‐displayed spores, is capable of capturing DNA targets for the detection of pathogenic bacteria and for mutation analysis in Wilson’s disease.</P><P><B>Significance and Impact of the Study: </B> This approach could be useful as a simple platform for developing sensitive spore‐based biosensors for any desired DNA targets in diagnostic applications.</P>