Pseudomonas veronii KJ mitigates flood stress-associated damage in Sesamum indicum L.

다국어 초록 (Multilingual Abstract)

Physiological characteristics of terrestrial plants are severely affected by waterlogging stress, leading to low photochemical efficiency of leaves and retarded growth and development. Plant growth-promoting rhizobacteria contain the acdS gene, which encodes for the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase.
ACC deaminase cleaves the substrate ACC to produce aketobutyrate and ammonia and mitigates the adverse effect of prolonged water stress. The aim of this study was to characterize ACC deaminase-producing rhizobacteria and evaluate their effects on sesame (Sesamum indicum L.) under waterlogging stress condition. The rhizobacterium Pseudomonas KJ was characterized on the basis of sequencing of the partial 1501 bp fragment of 16S rDNA amplicon and confirmed as Pseudomonas veronii KJ. ACCsupplemented minimal medium revealed the phenotypic identification of acdS gene. The nucleotide sequence (1001 bp) of ACC deaminase gene of P. veronii KJ was also confirmed. We used P. veronii KJ as a bioinoculant in waterlogging stress and monitored the growth and developmental characteristics of sesame plants, including leaf chlorophyll fluorescence signals, concentration of chlorophyll, root and shoot length, and fresh and dry biomass in stressed versus unstressed plants. Plants treated with P.
veronii KJ significantly (P B 0.05) mitigated the waterlogging stress-related damage. Thus, the rhizobacterium Pseudomonas veronii KJ may be considered as a commendable addition to the consortium of beneficial microbes for its ability to reduce waterlogging stress-related damage in sesame plants.

참고문헌 (Reference)

1 Farwell AJ, "Tolerance of transgenic canola plants (Brassica napus) amended with plant growth-promoting bacteria to flooding stress at a metal-contaminated field site" 147 (147): 540-545, 2007

2 Rogers ME, "The effect of saline irrigation on Lucerne production: shoot and root growth, ion relations and flowering incidence in six cultivars grown in Northern Victoria" 20 : 55-64, 2001

3 Gitelson AA, "The chlorophyll fluorescence ratio F735/F700 as an accurate measure of the chlorophyll content in plants" 69 (69): 296-302, 1999

4 Justin SHFW, "The anatomical characteristics of roots and plant response to soil flooding" 106 (106): 465-495, 1987

5 Sasidharan R, "Signal dynamics and interactions during flooding stress" 176 (176): 1106-1117, 2018

6 Lakhanpaul S, "Sesame: overcoming the abiotic stresses in the queen of oilseed crops" 1 (1): 1251-1283, 2012

7 Smethurst CF, "Screening methods for waterlogging tolerance in lucerne: comparative analysis of waterlogging effects on chlorophyll fluorescence, photosynthesis, biomass and chlorophyll content" 30 (30): 335-343, 2003

8 Hall JA, "Root elongation in various agronomic crops by the plant growth promoting rhizobacterium Pseudomonas putida GR12–2" 44 (44): 37-42, 1996

9 Glick BR, "Promotion of plant growth by bacterial ACC deaminase" 26 (26): 227-242, 2007

10 Amir Hossein Forghani, "Potential objectives for gibberellic acid and paclobutrazol under salt stress in sweet sorghum (Sorghum bicolor [L.] Moench cv. Sofra)" 한국응용생명화학회 61 (61): 113-124, 2018

11 Glick BR, "Plant growth-promoting bacteria: mechanisms and applications" 2012

12 Bjorkman O, "Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins" 170 (170): 489-504, 1987

13 Shaharoona B, "Performance of Pseudomonas spp. containing ACC-deaminase for improving growth and yield of maize (Zea mays L.) in the presence of nitrogenous fertilizer" 38 (38): 2971-2975, 2006

14 Martinchik AN, "Nutritional value of sesame seeds" 80 (80): 41-43, 2011

15 Couch A, "Non-dehiscent sesame (Sesamum indicum L.): its unique production potential and expansion into the southeastern USA" 31 (31): 101-172, 2017

16 Wei W, "Morpho-anatomical and physiological responses to waterlogging of sesame (Sesamum indicum L.)" 208 : 102-111, 2013

17 Penrose DM, "Methods for isolating and characterizing ACC deaminase containing plant growth promoting rhizobacteria" 118 (118): 10-15, 2003

18 Honma M, "Metabolism of 1-aminocyclopropane-1-carboxylic acid" 43 : 1825-1831, 1978

19 Tamura K, "MEGA6: molecular evolutionary genetics analysis version 6.0" 30 (30): 2725-2729, 2013

20 Valentini R, "In situ estimation of net CO2 assimilation, photosynthetic electron flow and photorespiration in Turkey oak (Q. cerris L.)leaves: diurnal cycles under different levels of water supply" 18 (18): 631-640, 1995

21 Li J, "Glick BR(2000)An ACC deaminase minus mutant of Enterobacter cloacae UW4No longer promotes root elongation" 41 (41): 101-105, 2000

22 Giovannoni SJTB, "Genetic diversity in Sargasso Sea bacterioplankton" 345 : 60-63, 1990

23 이창희, "Functional characterization of a chemical defoliant that activates fruit cluster Leaf defoliation in ‘Fuji’ apple trees" 한국응용생명화학회 59 (59): 711-720, 2016

24 Dworkin M, "Experiments with some microorganisms which utilize ethane and hydrogen" 75 : 592-601, 1958

25 Ihsan Ullah, "Exogenous ascorbic acid mitigates flood stress damages of Vigna angularis" 한국응용생명화학회 60 (60): 603-614, 2017

26 Nascimento F, "Enhanced chickpea growth-promotion ability of a Mesorhizobium strain expressing an exogenous ACC deaminase gene" 353 (353): 221-230, 2012

27 Morales-Olmedo M, "Effects of transient soil waterlogging and its importance for rootstock selection" 75 : 45-56, 2015

28 Castonguay Y, "Effects of flooding on carbohydrate and ABA levels in roots and shoots of alfalfa" 16 : 695-702, 1993

29 Kalaji HM, "Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions" 38 (38): 102-, 2016

30 Singh RP, "Biochemistry and genetics of ACC deaminase: a weapon to ‘‘stress ethylene’’produced in plants" 6 : 937-, 2015

31 Buchanan E, "Bergeys manual of determinative bacteriology" 24 : 607-616, 1994

32 Glick BR, "Bacteria with ACC deaminase can promote plant growth and help to feed the world" 169 (169): 30-39, 2014

33 Grichko VP, "Amelioration of flooding stress by ACC deaminase-containingplant growth-promoting bacteria" 39 (39): 11-17, 2001

34 Khandelwal A, "ACC deaminase containing rhizobacteria enhance nodulation and plant growth in Clusterbean (Cyamopsis tetragonoloba L.)" 3 (3): 117-123, 2013

35 Glick BR, "A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria" 190 (190): 63-68, 1998

36 Drancourt M, "16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates" 38 (38): 3623-3630, 2000

37 Glick BR, "1-Aminocyclopropane-1-carboxylic acid deaminase mutants of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 do not stimulate canola root elongation" 40 (40): 911-915, 1994