Characterization of Trichoderma spp. Associated with Green Mold of Oyster Mushroom by PCR-RFLP and Sequence Analysis of ITS Regions of rDNA

다국어 초록 (Multilingual Abstract)

Molecular profiles of PCR-RFLP and sequence analysis of internal transcribed spacer (ITS) regions of rDNA were compared between morphologically distinguishable species of Trichoderma isolated from substrates of oyster mushroom in Korea, T. atroviride, T. citrinoviride,T. harzianum, T. longibrachiatum, T. virens, and two unidentified species, Trichoderma sp. 1 and 2. PCRRFLP analysis divided the Trichoderma spp. into six RFLP groups, A, B, C, D, E, and F. The RFLP groups were generally agreed with described morphological species, except that the RFLP group A containing the two unidentified species. A neighbor-joining tree based on ITS sequences well supported RFLP groups observed by RFLP analysis of ITS regions of rDNA.Additionally, the two unidentified species, Trichoderma sp. 1 and 2, which could not be distinguished by PCRRFLP analysis, were separated in sequence analysis of ITS regions of rDNA.

참고문헌 (Reference)

1 "flexiblestrategies for multiple sequence alignment aided by qualityanalysis tools" 4876-4882, 1997

2 "What exactly is Trichoderma harzianum" 904-90 915, 1998

3 "Use of the RAPD procedure for the identification of Trichodermastrains" 531-534, 1994

4 "University of Newcastle" 1995

5 "UP-PCR analysis and ITS1 ribotyping ofstrains of Trichoderma and Gliocladium" 933-943, 1998

6 "Trichoderma species associated with the greenmold epidemic of commercially grown Agaricus bisporus" 146-94 170, 2002

7 "Revision of Trichoderma sect Longibrachiatumincluding related teleomorphs based on analysisof ribosomal DNA internal transcribed spacer sequences" 442-89 460, 1997

8 "Phylogeny of the genus Trichoderma based on sequenceanalysis of the internal transcribed spacer region 1 of therDNA cluster" 298-309, 1998

9 "Phylogeny and evolution of the genus Trichoderma" 757-767, 2002

10 "Phylogenetic relationships of Trichodermaharzianum causing mushroom green mold in Europeand North America to other species of Trichoderma fromworld-wide sources" 76-90 81, 1998

11 "PCR-fingerprintingused for comparison of ex-type strains of Trichoderma speciesdeposited in different culture collections" 1-9, 1995

12 "Morphologicaland macromolecular charaterization of Hypocrea schweinitziiand its Trichoderma anamorph" 421-86 435, 1994

13 "Morphological and cultural characteristics of Trichodermaspp associated with green mold of oyster mushroom in Korea" 221-228, 2005

14 "Molecular evidence that the asexual industrial fungusTrichoderma reesei is a clonal derivative of the ascomyceteHypocrea jecorina" 7755-93 7760, 1996

15 "Intraspecific molecular variation among Trichodermaharzianum isolates colonising mushroom compost in the BritishIsles" 769-140 777, 1994

16 "Fingerprinting reveals gamma-ray inducedmutations in fungal DNA implication for identification ofpatent strains of Trichoderma harzianum" 74-78, 1994

17 "Evalution of phenetic species and phylogeneticrelationships in the genus Trichoderma by cladistic analysis ofisozyme polymorphism" 391-81 403, 1989

18 "D W K Lieckfeldt E B Arisan-Atac J G J B？rner T andKubicek C P Biogeography and phenotypic" 449-459, 1997

19 "Confidence limits on phylogenies" tion 39 : 783-791, 1985

20 "Computer-aided objectivecomparison of electrophoresis patterns for grouping and identificationof microorganisms" 37-41, 1992

21 "Application of the randomamplified polymorphic DNA using the polymerase chain reactionfor efficient elimination of duplicate strains in microbialscreening I Fungi" 173-182, 1994

22 "Amplificationand direct sequencing of fungal ribosomal RNA genes forphylogenetics A guide to methods andapplication" Academic Press 315-322, 1990

23 "A simple method for estimating evolutionaryrate of base substitution through comparative studies of nucleotidesequence" e : 111-120, 1980

24 "A revision of the genus Trichoderma Additionalnotes on section Longibrachiatum" 2418-2420, 1991c

25 "A revision of the genus Trichoderma" 2373-2417, 1991b

26 "A revision of the genus Trichoderma" 2357-2372, 1991a

27 "A revision of the genus Trichoderma" (v) : 924-931, 1984

28 "A revision of the genus Trichoderma" 1-116 116, 1969