耐鹽性 煙草細胞群의 選拔 = Selection of Salt-tolerant Cell Line in Nicotiana tabacum

다국어 초록 (Multilingual Abstract)

Soil salinity by irrigation is a serious problem to agriculture leading to the decrease of crop production. To overcome this diffoculty, it is essential to adapt plants to tolerare saline enviornments.
The objective of this study was to select salt-tolerant cell line in tobacco through plant tissue culture techniques.
The summarized results were as follows.
Flue-cured tobacco(BY 4) callus was more tolerant and healthier to saline enviornment than that of air-cured (Burley 21).
The ratio of D.W./F.W. in callus was elevated in the presence of increasing NaCl concentrations.
Na^+ and Cl^- uptake was considerably larger in callus cultured on 200mM NaCl medium than that of NaCl-free. On the contrary, the amount of K^+ showed a slight decrease with external NaCl concentration.
Fresh weight showed a steady increase for 7 weeks and subsrquently declined in all but 200mM NaCl concentration.
The effect of NaCl on the regeneration of callus was negative.

목차 (Table of Contents)

• Ⅰ. 序 說
• Ⅱ. 材料 및 方法
• Ⅲ. 結果 및 考察
• Ⅳ. 結 論