The resistance‐nodulation‐division (RND)‐type efflux pumps AcrAB and MdtABC contribute to multidrug‐resistance (MDR) in Gram‐negative bacteria. Photorhabdus is a symbiotic bacterium of soil nematodes that also produces virulence factors kill...
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https://www.riss.kr/link?id=O112855518
2021년
-
1758-2229
SCOPUS;SCIE
학술저널
637-648 [※수록면이 p5 이하이면, Review, Columns, Editor's Note, Abstract 등일 경우가 있습니다.]
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
The resistance‐nodulation‐division (RND)‐type efflux pumps AcrAB and MdtABC contribute to multidrug‐resistance (MDR) in Gram‐negative bacteria. Photorhabdus is a symbiotic bacterium of soil nematodes that also produces virulence factors kill...
The resistance‐nodulation‐division (RND)‐type efflux pumps AcrAB and MdtABC contribute to multidrug‐resistance (MDR) in Gram‐negative bacteria. Photorhabdus is a symbiotic bacterium of soil nematodes that also produces virulence factors killing insects by septicaemia. We previously showed that mdtA deletion in Photorhabdus laumondii TT01 resulted in no detrimental phenotypes. Here, we investigated the roles of the last two putative RND transporters in TT01 genome, AcrAB and AcrAB‐like (Plu0759‐Plu0758). Only ΔacrA and ΔmdtAΔacrA mutants were multidrug sensitive, even to triphenyltetrazolium chloride and bromothymol blue used for Photorhabdus isolation from nematodes on the nutrient bromothymol blue‐triphenyltetrazolium chloride agar (NBTA) medium. Both mutants also displayed slightly attenuated virulence after injection into Spodoptera littoralis. Transcriptional analysis revealed intermediate levels of acrAB expression in vitro, in vivo and post‐mortem, whereas its putative transcriptional repressor acrR was weakly expressed. Yet, plasmid‐mediated acrR overexpression did not decrease acrAB transcript levels neither MDR in TT01 WT. While no pertinent mutations were detected in acrR of the same P. laumondii strain grown either on NBTA or nutrient agar, we suggest that AcrR‐mediated repression of acrAB is not physiologically required under conditions tested. Finally, we propose that AcrAB is the primary RND‐efflux pump, which is essential for MDR in Photorhabdus and may confer adaptive advantages during insect infection.
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