편백나무(Chamaecyparis obtusa) 추출물의 항산화, 미백효과에 관한 연구 = Studies on the Antioxidant and Whitening Effects of Chamaecyparis obtusa Extract

국문 초록 (Abstract)

본 연구는 화장품 소재로서 편백나무(Chamaecyparis obtusa)의 부위별 이용 가능성을 알아 보기 위하여 잎·가지(Leaf), 수피(Bark), 목재(Wood), 뿌리(Root)로 분리하여 99.9 % 에탄올에 추출한 시료로 항산화, 미백효능을 확인하였다. 이를 위해 다음과 같은 연구를 진행하였다. 항산화 평가는 1 1-diphenyl-2-picrylhydrazyl (DPPH) 라디칼 소거능, 2 2′-azino-bis (3-ethylbenzothiazoline--sulfonic acid) diammonium salt (ABTS + ) 라디칼 소거능으로 확인하였다. 미백 효과를 평가하기 위해서 mushroom tyrosinase 저해 활성 평가, MTT assay를 통한 편백나무 추출물의 세포독성평가를 진행 하였으며 cellular tyrosinase 저해율 측정과 melanin 생합성량을 확인하였다. 항산화 활성 결과 편백나무 부위 중 수피(Bark) 추출물이 가장 효능이 우수하였으며 B16F10 melanoma cell내 편백나무 수피(Bark) 는 tyrosinase 활성 억제 및 멜라닌 생합성 억제 효과를 나타내었다. 따라서 편백나무 수피 에탄올 추출 물의 안전하고 우수한 미백 기능성 화장품의 천연물질 소재로서의 개발 가능성을 확인하였다.

다국어 초록 (Multilingual Abstract)

This study was conducted as follows in order to find out the possibility of using cypress tree (Chamaecyparis obtusa) by part as a cosmetic material. Chamaecyparis obtusa was separated leaves, bark, wood, and root, and extracted with 99.9% ethanol, and the antioxidant and whitening effects of the sample were confirmed. For this, the following studies were conducted. Antioxidant evaluation was confirmed by 1 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS + ) radical scavenging activity. To evaluate the whitening effect, mushroom tyrosinase inhibitory activity evaluation and cytotoxicity evaluation of Chamaecyparis obtusa extract through MTT assay were conducted, and cellular tyrosinase inhibition rate was measured and melanin contents was confirmed. As a result of antioxidant activity, Bark extract among Chamaecyparis obtusa parts showed the best efficacy, and bark in B16F10 melanoma cell showed tyrosinase activity inhibition and melanin contents inhibitory effects. Therefore, it was confirmed that the ethanol extract of Chamaecyparis obtusa was developed as a natural material for safe and excellent whitening functional cosmetics.

참고문헌 (Reference)

1 최인규, "편백정유의 항산화활성" 한국목재공학회 36 (36): 159-167, 2008

2 서경아, "편백의 Malassezia furfur 에 대한 항균 및 비듬성 두피 개선 효과" 한국피부과학연구원 13 (13): 285-293, 2015

3 김보경, "편백나무 추출액의 항균 및 항산화 활성" 한국생명과학회 29 (29): 785-791, 2019

4 차유림, "편백나무 추출물이 아토피 유아의 피부 개선에 미치는 효과" 한국인체미용예술학회 13 (13): 75-87, 2012

5 김정희, "편백나무 잎 추출물의 성분분석과 면역효능에 관한 연구" 대한임상검사과학회 50 (50): 37-43, 2018

6 김재우, "편백 잎에서 추출한 정유와 초임계 이산화탄소추출물의 성분 비교분석" 한국목재공학회 43 (43): 494-503, 2015

7 정수지, "열풍건조 삼채의 분획별 미백 및 항산화 활성에 관한 연구" 한국식품영양과학회 44 (44): 832-839, 2015

8 정영태, "소태나무 잎 및 편백나무 추출물의 항산화 효과" 한국생명과학회 22 (22): 354-359, 2012

9 김선홍, "삼나무와 편백나무 정유의 미백 및 항산화 효능 평가" 한국목재공학회 39 (39): 291-302, 2011

10 최소영, "멜라닌 세포에서 홍차 열수추출물의 멜라닌 합성 저해능과 작용기전" 한국현미경학회 41 (41): 169-177, 2011

11 S. Parvez, "Survey and mechanism of skin depigmenting and lightening agents" 20 (20): 921-934, 2006

12 T. Hieda, "Sesquiterpene alcohols from Chamaecyparis obtusa leaf oil" 42 (42): 159-162, 1996

13 L. Z. Piao, "Mushroom tyrosinase inhibition activity of some chromones" 50 (50): 309-311, 2002

14 H. Ando, "Melanosomes Are Transferred from Melanocytes to Keratinocytes through the Processes of Packaging, Release, Uptake, and Dispersion" 132 (132): 1222-1229, 2012

15 J. P. Ebanks, "Mechanisms regulating skin pigmentation : the rise and fall of complexion coloration" 10 (10): 4066-4087, 2009

16 A. Korner, "Mammalian tyrosinase catalyzes three reactions in the bio synthesis of melanin" 217 (217): 1163-1165, 1982

17 K. Jimbow, "Increased sensitivity of mela nocytes to oxidative stress and abnormal expression of tyrosinase‐related protein in vitiligo" 144 (144): 55-65, 2001

18 K. Fujimori, "Hinokitiol (β-Thujaplicin) from the Essential Oil of Hinoki [Chamaecyparis obtusa (Sieb. et Zucc.) Endl.]" 10 (10): 711-712, 1998

19 C. U. Hong, "Composition of Essential Oils from the Leaves and the Fruits of Chamaecyparis obtusa and Chamaecyparis pisifera" 44 (44): 116-121, 2001

20 S. Del bino, "Clinical and Biological Characterization of Skin Pigmentation Diversity and Its Consequences on UV Impact" 19 (19): 2668-2712, 2018

21 J. G. Park, "Chemosensitivity testing of human colorectal carcinoma cell lines using a tetrazolium-based colorimetric assay" 47 (47): 5875-5879, 1987

22 H. Ando, "Approaches to identify inhibitors of melanin biosynthesis via the quality control of tyrosinase" 127 (127): 751-761, 2007

23 G. D. Lee, "Antioxidative and nitrite-scavenging activities of edible mushrooms" 29 (29): 432-436, 1997

24 K. Maeda, "A novel melanin inhibitor: hydroperoxy traxastanetype triterpene from flowers of Arnica montana" 30 (30): 873-879, 2007

25 F. A. Badria, "A new type of tyrosinase inhibitors from natural products as potential treatments for hyperpigmentation" 140 (140): 267-271, 2007