HPLC-MS/MS를 이용한 벌꿀 중 동물용 의약품 동시분석방법 연구 = Simultaneous Analysis for Veterinary Drug Residues in Honey by HPLC/MS/MS

다국어 초록 (Multilingual Abstract)

This study was conducted to establish the simultaneous analysis method for veterinary drug residues in honey by high performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS). The eleven targeting veterinary drugs with honey test method in Korean Food Standards Codex were divided into Group 1 (streptomycine dihydrostreptomycine, neomycine) and Group 2 (oxytetracycline, enrofloxacin, ciprofloxacin, cymiazole, chloramphenicol, amitraz, coumaphos, fluvalinate) to be analyzed simultaneously. From the results, the retention time(RT) of the targeting drugs was within 15 min, the range of detection limits was 0.0056 to 0.0643 μg/g and the range of quantification limits was 0.0169 to 0.1948 μg/g. The coefficients of determination(R2) for Group 1 (0.05~1.0 μg/mL) and Group 2 (0.01~1.0 μg/mL) were 0.9917~0.9987 and 0.9923~1.000 respectively, and showed the good linearity. The recovery rates for Group 1 (final conc. 0.25 μg/g) and Group 2(final conc. 1.0 μg/g) were 65.1~80.6% and 64.2~90.3% respectively. Also, the analysis results of inter day(n=3) and intra day(n=6) RSD(%) for area and retention time showed that the RSD(%) for area and retention time was below 10.92% and 1.57%. Therefore, the simultaneous analysis method of this study is evaluated to be a good test method for veterinary drug residues in honey.

참고문헌 (Reference)

1 McLaughlin L. G., "Multi-residue confirmation of aminoglycoside antibiotics and bovine kidney by ion spray high performance liquid chromatography/tandem mass spectrometry" 23 (23): 417-429, 1994

2 Won S. Y., "Monitoring of acaricide residues in honey." 11 : 189-201, 2007

3 MFDS, "Korean food standards codex Annex 7. Maximum residue limits of veterinary drugs in food"

4 MFDS, "Hazardous substances analysis of livestock and seafood products" Ministry of Food and Drug Safety 286-288, 2013

5 Rial-Otero R., "Gas chromatography mass spectrometry determination of acaricides from honey after a new fast ultrasonic-based solid phase micro-extraction sample treatment" 71 : 1906-1914, 2007

6 McLaughlin L., "Determination of aminoglycoside antibiotics by reversed phase ion-pair high performance liquid chromatography coupled with pulsed amperometry and ion spray mass spectrometry" 591 : 195-206, 1992

7 Kaufmann A., "Determination of 11 aminoglycosides in meat and liver by liquid chromatography with tandem mass spectrometry" 88 (88): 1118-1125, 2005

8 Heller D. N., "Confirmation of gentamicin and neomycin in milk by weak cation-exchange extraction and electrospray ionization/ion trap tandem mass spectrometry" 35 (35): 38-49, 2000

9 Jo D.H., "Beekeeping management for four seasons" Osung publisher 30-36, 2009