Effect of Scenedesmus sp. CHK0059 on Strawberry Microbiota Community

다국어 초록 (Multilingual Abstract)

Microalgae are photosynthetic cyanobacteria and eukaryotic microorganisms, mainly living in the water. In agriculture, numerous studies have been conducted to utilize microalgae as a biostimulant resource. Scenedesmus has been known to be one such microalga that can promote plant growth by secretion of auxin or cytokinin hormone analogs. However, no research has been performed on the effect of microalgae treatment on plant microbiota communities. This study was conducted to investigate the mode of action of microalgae as biostimulants in a plant microbiota perspective by using Scenedesmus sp. CHK0059 (also known as species Chlorella fusca), which has been well documented as a biostimulant for strawberries. The strawberry cultivar Keumsil was bred with Seolhyang and Maehyang as the parent cultivars. Using these three cultivars, microbiota communities were evaluated for changes in structural composition according to the CHK0059 treatment. CHK0059-treated Seolhyang, and CHK0059-untreated Maehyang were similar in microbial diversity in the endosphere. From a microbiota community perspective, the diversity change showed that CHK0059 was affected by the characteristics of the host. Conversely, when CHK0059 treatment was applied, populations of Streptomyces and Actinospica were observed in the crown endosphere.

참고문헌 (Reference)

1 김수정 ; 고은주 ; 홍점규 ; 전용철, "Ultrastructures of Colletotrichum orbiculare in Cucumber Leaves Expressing Systemic Acquired Resistance Mediated by Chlorella fusca" 한국식물병리학회 34 (34): 113-120, 2018

2 Martini F, "The potential use of Chlamydomonas reinhardtii and Chlorella sorokiniana as biostimulants on maize plants" 60 : 102515-, 2021

3 Dineshkumar R, "The impact of using microalgae as biofertilizer in maize (Zea mays L.)" 10 : 1101-1110, 2019

4 Kublanovskaya A, "The effect of the microalga Chlorella vulgaris Ippas C-1 biomass application on yield, biological activity, and the microbiome of the soil during bean growing" 74 : 227-234, 2019

5 Nedelcu AM, "The complete mitochondrial DNA sequence of Scenedesmus obliquus reflects an intermediate stage in the evolution of the green algal mitochondrial genome" 10 : 819-831, 2000

6 Bulgarelli D, "Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota" 488 : 91-95, 2012

7 Rana A, "Prospecting plant growth promoting bacteria and cyanobacteria as options for enrichment of macro-and micronutrients in grains in rice–wheat cropping sequence" 1 : 1037379-, 2015

8 Zulma Rocío Suárez-Moreno, "Plant-Growth Promotion and Biocontrol Properties of Three Streptomyces spp. Isolates to Control Bacterial Rice Pathogens" Frontiers Media SA 10 : 2019

9 Lee SM, "Plant anti-aging : delayed flower and leaf senescence in Erinus alpinus treated with cell-free Chlorella cultivation medium" 15 : 1763005-, 2020

10 Kessler E, "Physiological, biochemical, and molecular characters for the taxonomy of the subgenera of Scenedesmus(Chlorococcales, Chlorophyta)" 110 : 244-250, 1997

11 Cha JY, "Microbial and biochemical basis of a Fusarium wilt-suppressive soil" 10 : 119-129, 2016

12 Rosenberg E, "Microbes drive evolution of animals and plants : the hologenome concept" 7 : e01395-15, 2016

13 Renuka N, "Microalgae as multi-functional options in modern agriculture : current trends, prospects and challenges" 36 : 1255-1273, 2018

14 Murali A, "IDTAXA : a novel approach for accurate taxonomic classification of microbiome sequences" 6 : 140-, 2018

15 Kim DR, "Glutamic acid reshaphes the plant microbiota to protec plants against pathogens" 9 : 244-, 2021

16 김민정 ; 심창기 ; 고병구 ; 김주, "Effect of the Microalga Chlorella fusca CHK0059 on Strawberry PGPR and Biological Control of Fusarium Wilt Disease in Non-Pesticide Hydroponic Strawberry Cultivation" 한국미생물·생명공학회 30 (30): 708-716, 2020

17 Sang‐Moo Lee, "D‐lactic acid secreted by Chlorella fusca primes pattern‐triggered immunity against Pseudomonas syringae in Arabidopsis" Wiley 102 (102): 761-778, 2020

18 Callahan BJ, "DADA2 : high-resolution sample inference from Illumina amplicon data" 13 : 581-583, 2016

19 Righini H, "Cyanobacteria : a natural source for controlling agricultural plant diseases caused by fungi and oomycetes and improving plant growth" 8 : 58-, 2022

20 Searchinger T, "Creating a sustainable food future: a menu of solutions to feed nearly 10 billion people by 2050" World Resources Institute 2019

21 Elvira Navarro-López, "Biostimulant Potential of Scenedesmus obliquus Grown in Brewery Wastewater" MDPI AG 25 (25): 664-, 2020

22 Lin H, "Analysis of compositions of microbiomes with bias correction" 11 : 3514-, 2020

23 Lee SM, "Algae as new kids in the beneficial plant microbiome" 12 : 599742-, 2021

24 Compant S, "A review on the plant microbiome : ecology, functions, and emerging trends in microbial application" 19 : 29-37, 2019

25 Kim DR, "A mutualistic interaction between Streptomyces bacteria, strawberry plants and pollinating bee" 10 : 4802-, 2019

26 Wu Y, "A field study reveals links between hyperaccumulating Sedum plants-associated bacterial communities and Cd/Zn uptake and translocation" 805 : 150400-, 2022

27 김다란 ; 곽연식, "A Genome-Wide Analysis of Antibiotic Producing Genes in Streptomyces globisporus SP6C4" 한국식물병리학회 37 (37): 389-395, 2021