Identifying a Candidate Mutation Underlying a Reduced Cuticle Wax Mutant of Rice Using Targeted Exon Capture and Sequencing

다국어 초록 (Multilingual Abstract)

Aerial surfaces of terrestrial plants are protected from the uncontrolled loss of water and gas by the cuticle, a membrane of fatty acid polymers on the outer surface of epidermal cells. Composed of cutin and waxes, the cuticle protects against a wide range of external stresses and has an important role in plant development and reproduction. Plants with reduced cuticular waxes often exhibit glossy, bright green leaves, which in rice are only observed in the presence of water adhesion. In this study, a wet leaf/glossy (wlg) mutant KDS-2249D was subjected to targeted exon capture and sequencing to identify candidate mutations. A single nonsynonymous, homozygous mutation was found in the KDS-2249D mutant. The mutation (G1080A) is predicted to change a tryptophan at position 360 to a stop codon in the Glossy1-like-1/wax crystal-sparse leaf 2 gene. This mutation completely co-segregated with the wlg phenotype in an F2 mapping population (n = 435) and the KDS-2249D mutant exhibited a 40-50% decrease in total wax and significant increase in membrane permeability. This mutant will be useful for studies examining the role of cuticle waxes in protecting rice plants from environmental stresses.

참고문헌 (Reference)

1 Jung K-H, "Wax-deficient anther1 is involved in cuticle and wax production in rice anther walls and is required for pollen development" 18 : 3015-3032, 2006

2 Mao B, "Wax crystal-sparse leaf2, a rice homologue of WAX2/GL1, is involved in synthesis of leaf cuticular wax" 235 : 39-52, 2012

3 Yu D, "Wax crystal-sparse leaf1 encodes a -ketoacyl CoA synthase involved in biosynthesis of cuticular waxes on rice leaf" 228 : 675-685, 2008

4 Gan L, "Wax crystal-sparse leaf 3 encoding a -ketoacyl-CoA reductase is involved in cuticular wax biosynthesis in rice" 35 : 1687-1698, 2016

5 Gan L, "Wax Crystal-Sparse Leaf 4, encoding a -ketoacylcoenzyme A synthase 6, is involved in rice cuticular wax accumulation" 36 : 1655-1666, 2017

6 Sieber P, "Transgenic Arabidopsis plants expressing a fungal cutinase show alterations in the structure and properties of the cuticle and postgenital organ fusions" 12 : 721-738, 2000

7 Baker EA., "The plant cuticle" Academic Press 139-165, 1982

8 Yeats TH, "The formation and function of plant cuticles" 163 : 5-20, 2013

9 Serrano M, "The cuticle and plant defense to pathogens" 5 : 274-, 2014

10 Alexandrov N, "SNP-Seek database of SNPs derived from 3000 rice genomes" 43 : D1023-D1027, 2014

11 Mansueto L, "Rice SNP-seek database update : new SNPs, indels, and queries" 45 : D1075-D1081, 2017

12 Zhou L, "Rice OsGL1-6 is involved in leaf cuticular wax accumulation and drought resistance" 8 : e65139-, 2013

13 Qin B-X, "Rice OsGL1-1 is involved in leaf cuticular wax and cuticle membrane" 4 : 985-995, 2011

14 Tseng ST, "Registration of L-202 Rice" 24 : 1213-1214, 1984

15 Monson-Miller J, "Reference genome-independent assessment of mutation density using restriction enzymephased sequencing" 13 : 72-, 2012

16 Zhu X, "Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax deposition in rice" 110 : 17790-17795, 2013

17 Krogh A, "Predicting transmembrane protein topology with a hidden markov model: application to complete genomes" 305 : 567-580, 2001

18 Clark JB, "Photosynthetic action spectra of trees: II. The relationship of cuticle structure to the visible and ultraviolet spectral properties of needles from four coniferous species" 55 : 407-413, 1975

19 Zhou X, "Overexpression of transcription factor OsWR2 regulates wax and cutin biosynthesis in rice and enhances its tolerance to water deficit" 32 : 719-731, 2013

20 Xia K, "OsWS1 involved in cuticular wax biosynthesis is regulated by osa-miR1848" 38 : 2662-2673, 2015

21 Zhang Z, "OsHSD1, a hydroxysteroid dehydrogenase, is involved in cuticle formation and lipid homeostasis in rice" 249 : 35-45, 2016

22 Zhou X, "OsGL1-3 is involved in cuticular wax biosynthesis and tolerance to water deficit in rice" 10 : e116676-, 2015

23 Kim S-I, "Identification of novel rice low phytic acid mutations via TILLING by sequencing" 34 : 1717-1729, 2014

24 Tai TH, "Identification and characterization of reduced epicuticular wax mutants in rice" 22 : 171-179, 2015

25 Nguyen VNT, "Genome-wide expression analysis of rice ABC transporter family across spatio-temporal samples and in response to abiotic stresses" 1276-1288, 171

26 Kearse M, "Geneious Basic : an integrated and extendable desktop software platform for the organization and analysis of sequence data" 28 : 1647-1649, 2012

27 Lolle SJ, "Fiddlehead: An Arabidopsis mutant constitutively expressing an organ fusion program that involves interactions between epidermal cells" 152 : 383-392, 1992

28 Guo L, "Evaluating the microtubule cytoskeleton and its interacting proteins in monocots by mining the rice genome" 103 : 387-402, 2009

29 Henry IM, "Efficient genome-wide detection and cataloging of EMS-induced mutations using exome capture and next-generation sequencing" 26 : 1382-1397, 2014

30 Kharabian-Masouleh A, "Discovery of polymorphisms in starch-related genes in rice germplasm by amplification of pooled DNA and deeply parallel sequencing" 9 : 1074-1085, 2011

31 Jain M, "Comprehensive expression analysis suggests overlapping and specific roles of rice glutathione S-transferase genes during development and stress responses" 11 : 73-, 2010

32 Jenks MA, "Chemically induced cuticle mutation affecting epidermal conductance to water vapor and disease susceptibility in Sorghum bicolor(L.)Moench" 105 : 1239-1245, 1994

33 Islam MA, "Characterization of Glossy1-homologous genes in rice involved in leaf wax accumulation and drought resistance" 70 : 443-456, 2009

34 Post-Beittenmiller D, "Biochemistry and molecular biology of wax production in plants" 47 : 405-430, 1996

35 Bernard A, "Arabidopsis cuticular waxes: advances in synthesis, export and regulation" 52 : 110-129, 2013

36 Riederer M., "Annual plant reviews, volume 23" Blackwell Pub 1-8, 2006

37 Kim SL, "Analysis of the early-flowering mechanisms and generation of T-DNA tagging lines in Kitaake, a model rice cultivar" 64 : 4169-4182, 2013

38 Wang Y, "An ethylene response factor OsWR1 responsive to drought stress transcriptionally activates wax synthesis related genes and increases wax production in rice" 78 : 275-288, 2012

39 Fiebig A, "Alterations in CER6, a gene identical to CUT1, differentially affect long-chain lipid content on the surface of pollen and stems" 12 : 2001-2008, 2000

40 Koornneef M, "A genetic and phenotypic description of eceriferum (cer) mutants in Arabidopsis thaliana" 80 : 118-122, 1989

41 Preuss D, "A conditional sterile mutation eliminates surface components from Arabidopsis pollen and disrupts cell signaling during fertilization" 7 : 974-985, 1993