액체배양된 P aecilomyces japonica로부터 cordycepin 분리를 위한 분획용매의 영향 = Effect of Fraction Solvent for Isolation of Cordycepin from Submerged Cultured P aecilomyces japonica

다국어 초록 (Multilingual Abstract)

Cordycepin is a nucleoside analog (3′-deoxyadenosine), which is the major active constituent of Cordyceps militaris and was first reported as a metabolite isolated from a culture broth of P. japonica. To date, cordycepin has been demonstrated to have many pharmacological activities, such as anti-tumor, anti-metastatic immunomodulatory and anti-inflammatory activities, and supply of a large amount of purified cordycepin is essential to the developments of its novel pharmacological use as well as its clinical trials. However, there are very few reports on commercially feasible procedures for isolation and purification of cordycepin. From the discovery of corycepin to the present, the purification methods have been based on column chromatography.
The present study was carried out to develop an extraction strategy for cordycepin and to isolation of cordycepin from Paecilomyces japonica in submerged culture. Solvent-solvent extraction method was used to extract cordycepin from submerged culture of P. japonica. Crude concentrated extract of fermented broth was sequentially partitioned with hexane, chloroform and n-butanol. Further we tried to isolation of cordycepin from P. japonica using Sephadex-LH20. As a result, it was confirmed that cordycepin was detected when the liquid culture medium was fractionated using n-butanol. It was also confirmed that a single cordycepin was detected when separated by sephadex column chromatography using 80% methanol as a solvent. At this time, the detection of cordycepin was analyzed by HPLC. It is hoped that these results will be used as basic data for cordycepin separation from Cordyceps mycelia.