High-throughput proteome analysis reveals changes of primary metabolism and energy production under artificial aging treatment in Glycine max seeds

다국어 초록 (Multilingual Abstract)

This study was conducted to obtain basic information on protein profile changes by artificial aging in soybean seeds. Seed proteins were extracted using the protamine sulfate precipitation method, which improves the detection of low-abundance proteins (LAPs) by depleting the major seed storage proteins . Isolated proteins were separated by high-resolution two-dimensional gel electrophoresis (2-DE), and differentially modulated protein spots were identified by MALDI-TOF/TOF. A total of 33 differential proteins were identified of which 31 and 2 showed decreased and increased abundances, respectively. Functional annotation of the identified proteins revealed that proteins were mainly associated with primary metabolism (55%) and response to stimulus (20.9%). Proteins with increased abundance were associated with nutrient reservoir activity (spots 5, 10), while the decreased abundance proteins were mainly involved in the primary metabolism such as carbohydrate metabolic process (spots 1–3, 11), protein folding (spots 6–9, 33), glucose metabolic process (spot 25) oxidoreductase activity (spots 19–24), UDP-glucose pyrophosphorylase activity (spots 12, 13). These results provide information about proteome changes, especially, LAPs during artificial seed aging treatment.

참고문헌 (Reference)

1 Gupta R, "Time to dig deep into the plant proteome: a hunt for low-abundance proteins" 6 : 22-, 2015

2 Qi Q, "The utilization of glycolytic intermediates as precursors for fatty acid biosynthesis by pea root plastids" 107 : 413-419, 1995

3 Plaxton WC, "The organization and regulation of plant glycolysis" 47 : 185-214, 1996

4 Qiu Y, "The function of calreticulin in plant immunity: new discoveries for an old protein" 7 : 907-910, 2012

5 Kibinza S, "Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging" 128 : 496-506, 2006

6 Da Silva PMFR, "Starch metabolism in developing embryos of oilseed rape" 203 : 480-487, 1997

7 Wang W, "Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response" 9 : 244-252, 2004

8 Parkhey S, "ROS production and lipid catabolism in desiccating Shorea robusta seeds during aging" 57 : 261-267, 2012

9 Dong K, "Proteomic analysis reveals key proteins and phosphoproteins upon seed germination of wheat (Triticum aestivum L.)" 6 : 1017-, 2015

10 Rajjou L, "Proteome-wide characterization of seed aging in arabidopsis: a comparison between artificial and natural aging protocols" 148 : 620-641, 2008

11 Xin X, "Proteome analysis of maize seeds: the effect of artificial ageing" 143 : 126-138, 2011

12 Kim YJ, "Protamine sulfate precipitation method depletes abundant plant seed-storage proteins: a case study on legume plants" 15 : 1760-1764, 2015

13 Sharma S, "Positional effects on soybean seed composition during storage" 50 : 353-359, 2013

14 Sung D, "Plant Hsp70 molecular chaperones: protein structure, gene family, expression and function" 113 : 443-451, 2001

15 Yin X, "Physiological and proteomic analyses on artificially aged Brassica napus seed" 6 : 112-, 2015

16 Mahjabin Bilal S, "Physiological and biochemical changes during deed deterioration: a review" 6 : 3416-3422, 2015

17 Friedman M, "Nutritional value of proteins from different food sources. A review" 44 : 6-29, 1996

18 Jia XY, "Molecular cloning and characterization of wheat calreticulin (CRT) gene involved in drought-stressed responses" 59 : 739-751, 2008

19 Hartl FU, "Molecular chaperones in cellular protein folding" 381 : 571-579, 1996

20 Boston RS, "Molecular chaperones and protein folding in plants" 32 : 191-222, 1996

21 Murthy UMN, "Mechanisms of seed ageing under different storage conditions for Vigna radiata (L.) Wilczek: lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship to glass state transition" 54 : 1057-1067, 2003

22 Messina MJ, "Legumes and soybeans: overview of their nutritional profiles and health effects" 70 : 439-450, 1999

23 Scarpeci TE, "Investigating the role of plant heat shock proteins during oxidative stress" 3 : 856-857, 2008

24 Agrawal GK, "In-Depth investigation of the soybean seed-filling proteome and comparison with a parallel study of rapeseed" 148 : 504-518, 2008

25 Prieto-Dapena P, "Improved resistance to controlled deterioration in transgenic seeds" 142 : 1102-1112, 2006

26 Bailly C, "From intracellular signaling networks to cell death: the dual role of reactive oxygen species in seed physiology" 331 : 806-814, 2008

27 Gupta R, "Expect the unexpected enrichment of "Hidden Proteome" of seeds and tubers by depletion of storage proteins" 7 : 1-7, 2016

28 Shackelford GS, "Evolutionary trace analysis of the a-D-phosphohexomutase superfamily" 13 : 2130-2138, 2004

29 Anderson JD, "Deterioration of seeds during aging" 73 : 321-, 1983

30 Kim YJ, "Depletion of abundant plant RuBisCO protein using the protamine sulfate precipitation method" 13 : 2176-2179, 2013

31 Khan M, "Comprehensive phosphoproteome analysis in rice and identification of phosphoproteins responsive to different hormones/stresses" 4 : 1592-1599, 2005

32 Wang L, "Comparative proteomics analysis reveals the mechanism of pre-harvest seed deterioration of soybean under high temperature and humidity stress" 75 : 2109-2127, 2012

33 Kim YJ, "Comparative proteomics analysis of seed coat from two black colored soybean cultivars during seed development" 6 : 456-463, 2013

34 Gupta R, "Comparative investigation of seed coats of brown- versus yellow-colored soybean seeds using an integrated proteomics and metabolomics approach" 15 : 1706-1716, 2015

35 Min CW, "Comparative biochemical and proteomic analyses of soybean seed cultivars differing in protein and oil content" 63 : 7134-7142, 2015

36 Bentsink L, "Cloning of DOG1, a quantitative trait locus controlling seed dormancy in arabidopsis" 103 : 17042-17047, 2006

37 Murata M, "Chromosome damage induced by artificial seed aging in barley" 67 : 161-170, 1984

38 Natarajan SS, "Characterization of storage proteins in wild (Glycine soja) and cultivated (Glycine max) soybean seeds using proteomic analysis" 54 : 3114-3120, 2006

39 Bailly C, "Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging" 97 : 104-110, 1996

40 Jia XY, "Calreticulin: conserved protein and diverse functions in plants" 136 : 127-138, 2009

41 Gelebart P, "Calreticulin, a Ca2+-binding chaperone of the endoplasmic reticulum" 37 : 260-266, 2005

42 Pandey G, "Calcium homeostasis in plants: role of calcium binding proteins in abiotic stress tolerance" 1 : 135-157, 2002

43 Nagel M, "Barley seed aging: genetics behind the dry elevated pressure of oxygen aging and moist controlled deterioration" 7 : 388-, 2016

44 Kim SW, "An integrated biochemical, proteomics, and metabolomics approach for supporting medicinal value of panax ginseng fruits" 7 : 994-, 2016

45 Egli DB, "Air temperature during seed filling and soybean seed germination and vigor" 45 : 1329-1335, 2005

46 Torres RM, "Accelerated aging and seedling field emergence in soybean" 61 : 476-480, 2004