Statistical analysis and genetic diversity of three dog breeds using simple sequence repeats

다국어 초록 (Multilingual Abstract)

There are more than 400 pure dog breedsdeveloped through intentional artificial selection andpurebred breeding. Purebred animals have higher risk ofinbreeding depression and hereditary diseases. We investigatedthe genetic diversity and structure of three dogbreeds in South Korea by using 12 microsatellite loci forone Korean native dog breed, Sapsaree, and two foreignbreeds, German shepherd and Belgian Malinois. The meanallele number of nine loci across all dog breeds was 4.833,and the number of alleles per locus ranged from 2 to 8. Themean of expected and observed heterozygosity were 0.415and 0.577, respectively. Sapsaree, Korean native dog, hadhigher level of genetic diversity than the foreign Germanshepherd and Belgian Malinois. The highest mean value ofpolymorphism information content was found in Sapsaree(0.480), followed by Belgian Malinois (0.373) and Germanshepherd (0.355). Pairwise genetic differentiation wasestimated using fixation index FST. Sapsaree and Germanshepherd (FST = 0.2536) and Sapsaree and Belgian Malinois(FST = 0.2522) had very great genetic differentiation,while moderate level of genetic differentiation wasobserved between German shepherd and Belgian Malinois(FST = 0.1003). These genetic information and structure ofthe three dog breeds will be effective in conservation andpreservation of the genetic diversity of the three dogbreeds.

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