In Vitro Sugar Accumulation in Juice Sacs of ‘Shiranuhi’ Mandarin

다국어 초록 (Multilingual Abstract)

To further our understanding of sugar accumulation in ‘Shiranuhi’ mandarin [(C. unshiu × C. sinensis)× C. reticulate], we investigated the patterns of sugar uptake in juice sacs exposed to different concentrations of sucrose, fructose and glucose in vitro. Data was also collected on the change in weight and shape of the in vitro juice sacs over time. Soluble solids content, sugar content and acidity content were highest at 20% sucrose, fructose and glucose solution content; while fructose content was highest at 5% sucrose concentration. Furthermore, the juice sac’s fresh weight was highest at 5% sucrose and lowest at 20% fructose content. The shape of the juice sacs also differed in different sugar concentration and type. Overall, sucrose,fructose and glucose content in juice sacs increased with the sugar concentration. These results suggest that sugar translocation into juice sacs is actively induced by high sugar concentration in the medium. Thus, it can be concluded that sugar and acid accumulation in juice sacs increased with sugar concentration in vitro culture.

참고문헌 (Reference)

1 Matsumoto, R., "‘Shiranuhi’ a late-maturing citrus cultivar" 35 : 115-120, 2001

2 Tomlinson, P., "synthase and invertase in isolated vascular bundles" 97 : 1249-1252, 1991

3 olomew, E.T., "Unequal distribution of soluble solids in the pulp of citrus fruits" 16 : 293-312, 1941

4 Burns, J.K., "Ultrastructural studies on the ontogeny of grapefruit juice vesicles (Citrus paradisi Macf. cv. Star Ruby)" 153 : 14-25, 1992

5 문두경, "Sugars and Organic Acid Contents in Different Parts of Juice Sacs in ‘Shiranuhi’ Mandarin Fruit at Harvest" 한국원예학회 50 (50): 515-518, 2009

6 문두경, "Sugar and Acid Contents in Different Portions of ‘Shiranuhi’ Mandarin Fruit as Affected by Water Stress" 한국원예학회 49 (49): 216-220, 2008

7 Mukai, H., "Sugar accumulation in fruit of several satsuma mandarin cultivars" 69 : 624-628, 2000

8 Yakushiji, H., "Sugar accumulation enhanced by osmoregulation in satsuma mandarin fruit" 121 : 466-472, 1996

9 Yakushiji, H., "Sugar accumulation and partitioning in satsuma mandarin tree tissues and fruit in response to drought stress" 123 : 719-726, 1998

10 Lowell, C.A., "Sucrosemetabolizing enzymes in transport tissues and adjacent sink structures in developing citrus fruit" 90 : 1394-1402, 1989

11 Kubo, T., "Sucrose accumulation and its related enzyme activities in the juice sacs of satsuma mandarin fruit from trees with different crop loads" 91 : 215-225, 2001

12 Kadoya, K., "Studies on the translocation of photosynthates in satsuma mandarin. IV. Diurnal fluctuations of fruit growth and some environmental conditions" 42 : 215-220, 1973

13 Moon, D.G., "Relationship between fruit shape and acid content in different parts of citrus fruits" 71 : 56-58, 2002

14 Doo-Gyung Moon, "Partitioning Pattern of 13C-Assimilates in Different Portions of Maturing Satsuma Mandarin Fruit" 한국원예학회 48 (48): 32-35, 2007

15 Huberman, M., "In vitro sugar uptake by grapefruit (Citrus paradise) juice-sac cells" 32 : 357-366, 2005

16 Mukai, H., "Effects of sugar concentration and water potential in the medium on sugar accumulation by in vitro cultured juice vesicles of satsuma mandarin" 70 : 238-243, 2001

17 Song, K.J., "Distribution of sugars and related enzyme in the stem and blossom halves of ‘Valencia’ oranges" 123 : 416-420, 1998

18 Ting, S.V., "Distribution of soluble components and quality factors in the edible portion of citrus fruits" 94 : 515-519, 1969

19 Komatsu, A., "Differential expression of three sucrose-phosphate synthase isoforms during sucrose accumulation in citrus fruit (Citrus unshiu Marc.)" 140 : 169-178, 1999

20 Saunt, J., "Citrus varieties of the world" Sinclair Intl. Ltd 15-156, 2000

21 Spiegel-Roy, P., "Biology of citrus" Cambridge University Press 88-125, 1996

22 Nii, N., "Anatomical aspects of juice sacs of satsuma mandarin in relation to translocation" 56 : 375-381, 1988

23 Murashige, T., "A revised medium for rapid growth and bioassay with tobacco tissue cultures" 15 : 473-497, 1962