CTX-M-12와 새로운 CTX-M형 Extended-Spectrum β-Lactamase생성 Klebsiella pneumoniae의 출현 = Emergence of CTX-M-12 and A Novel CTX-M Type Extended-Spectrum β-LactamaseproducingKlebsiella pneumoniae

국문 초록 (Abstract)

배경 : 본 연구는 전국 12개병원에서 분리된 Klebsiella pneu-moniae를 대상으로 class A형 extended-spectrum -lactamase(ESBL)의 생성현황을조사하고자 하였다.방법 : 2004년 2-7월에 전국 12개 병원에서 분리된 ceftazidime이나 cefotaxime에 내성인K. pneumoniae 를 수집하였다. 항균제감수성을 디스크 확산법과 한천희석법으로 시험하였으며, ESBL생성은 double-disk synergy 시험으로 확인하였다. ESBL 생성균주의 내성을 접합으로 azide 내성 E. coli J53으로 전달하였다.PCR로 ESBL 유전자를 검출하였고, PCR 산물의 염기서열을 양방향으로 분석하였다.결과 : 전국 12개 병원에서 수집된 212주 중 172 (81%)주가double-disk synergy 양성이었다. 가장 흔한 ESBL은 SHV-12(104주)였으며, SHV-2(6주), SHV-2a (17주), CTX-M-3 (18주), CTX-M-9 (6주), CTX-M-12 (1주), CTX-M-14 (27주),CTX-M-15 (3주) 및 새로운 CTX-M형 유전자(1주)도 검출되었다.

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