Immune response of heterologous recombinant antigenic protein of viral hemorrhagic septicemia virus (VHSV) in mice

다국어 초록 (Multilingual Abstract)

Viral hemorrhagic septicemia (VHS) is an important infectious disease in fish worldwide caused by viral hemorrhagic septicemia virus (VHSV). VHSV is the causative agent of serious systemic diseases in fish, affecting a number of teleost fish species. In this study, VHSV glycoprotein (G), including its epitope, as a subunit vaccine candidate, was expressed in tobacco plant (Nicotiana tabacum). The recombinant gene, VHSVG, was fused to the immunoglobulin Fc fragment and extended with the endoplasmic reticulum (ER) retention signal (KDEL) to generate VHSVG-FcK. The recombinant expression vector for VHSVG-FcK was transferred into Agrobacterium tumefaciens (LBA4404), and plant transformation was conducted N. tabacum. Polymerase chain reaction (PCR) was performed to confirm gene insertion and VHSVG-FcK protein expression was confirmed by immunoblot analysis. VHSVG-FcK protein was successfully purified from tobacco plant leaves. Furthermore, ELISA analysis showed that mice serum immunized with the plant-derived VHSVG-FcK (VHSVGPFcK) had a high absorbance against VHSVG-FcK, indicating that the plant-derived recombinant subunit vaccine protein VHSVG-FcK can induce immune response. Taken together, this recombinant vaccine protein can be expressed in plant expression systems and can be appropriately assembled to be functional in immunogenicity.

참고문헌 (Reference)

1 Crisci E, "Virus-like particles : the new frontier of vaccines for animal viral infections" 148 : 211-225, 2012

2 Skall HF, "Viral haemorrhagic septicaemia virus in marine fish and its implications for fish farming – a review" 28 : 509-529, 2005

3 Kemper N, "Veterinary antibiotics in the aquatic and terrestrial environment" 8 : 1-13, 2008

4 Biering E, "Update on viral vaccines for fish" 121 : 97-113, 2005

5 De Meyer T, "Trafficking of endoplasmic reticulum-retained recombinant proteins is unpredictable in Arabidopsis thaliana" 5 : 473-, 2014

6 Coll J, "The glycoprotein G of rhabdoviruses" 140 : 827-851, 1995

7 Boxall AB, "The environmental side effects of medication:how are human and veterinary medicines in soils and water bodies affecting human and environmental health?" 5 : 1110-1116, 2004

8 Flanagan ML, "Soluble Fc fusion proteins for biomedical research" 378 : 33-52, 2007

9 Yin J, "Select what you need : a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes" 127 : 335-347, 2007

10 Rosano GL, "Recombinant protein expression in Escherichia coli : advances and challenges" 5 : 172-, 2014

11 이정환, "Production of Recombinant Anti-Cancer Vaccines in Plants" 한국응용약물학회 25 (25): 345-353, 2017

12 Gomord V, "Posttranslational modification of therapeutic proteins in plants" 7 : 171-181, 2004

13 Walsh G, "Post-translational modifications in the context of therapeutic proteins" 24 : 1241-, 2006

14 Matzke AJ, "Position effects and epigenetic silencing of plant transgenes" 1 : 142-148, 1998

15 Sharma AK, "Plants as bioreactors : recent developments and emerging opportunities" 27 : 811-832, 2009

16 Gomord V, "Plant-specific glycosylation patterns in the context of therapeutic protein production" 8 : 564-587, 2010

17 Arcalis E, "Plant species and organ influence the structure and subcellular localization of recombinant glycoproteins" 83 : 105-117, 2013

18 이정환, "Murine response studies of insect cell (Sf9) expressed recombinant colorectal cancer vaccine candidate using surface plasmon resonance studies" 한국곤충학회 46 (46): 5-14, 2016

19 Twyman RM, "Molecular farming in plants : host systems and expression technology" 21 : 570-578, 2003

20 Lorenzen N, "Molecular cloning and expression in Escherichia coli of the glycoprotein gene of VHS virus, and immunization of rainbow trout with the recombinant protein" 74 : 623-630, 1993

21 Zhu J, "Mammalian cell protein expression for biopharmaceutical production" 30 : 1158-1170, 2012

22 Kooter JM, "Listening to the silent genes : transgene silencing, gene regulation and pathogen control" 4 : 340-347, 1999

23 Caipang CMA, "In vitro inhibition of fish rhabdoviruses by Japanese flounder, Paralichthys olivaceus Mx" 317 : 373-382, 2003

24 Lorenzen N, "Immunity to VHS virus in rainbow trout" 172 : 41-61, 1999

25 Cabello FC, "Heavy use of prophylactic antibiotics in aquaculture : a growing problem for human and animal health and for the environment" 8 : 1137-1144, 2006

26 Ko K, "Function and glycosylation of plant-derived antiviral monoclonal antibody" 100 : 8013-8018, 2003

27 Nimmerjahn F, "Fc-receptors as regulators of immunity" 96 : 179-204, 2007

28 Czajkowsky DM, "Fc-fusion proteins : new developments and future perspectives" 4 : 1015-1028, 2012

29 Lu Z, "Expression of GA733-Fc fusion protein as a vaccine candidate for colorectal cancer in transgenic plants" 2012 : 1-11, 2012

30 Walker PJ, "Emerging viral diseases of fish and shrimp" 41 : 51-, 2010

31 DiLillo DJ, "Differential Fc-receptor engagement drives an anti-tumor vaccinal effect" 161 : 1035-1045, 2015

32 Lorenzen N, "DNA vaccines for aquacultured fish" 24 : 201-, 2005

33 Brun A, "Current strategies for subunit and genetic viral veterinary vaccine development" 157 : 1-12, 2011

34 Tekoah Y, "Controlled glycosylation of therapeutic antibodies in plants" 426 : 266-278, 2004

35 Schu H, "Complete genomic sequence of viral hemorrhagic septicemia virus, a fish rhabdovirus" 19 : 59-65, 1999

36 Byon JY, "Comparative immune responses in Japanese flounder, Paralichthys olivaceus after vaccination with viral hemorrhagic septicemia virus(VHSV)recombinant glycoprotein and DNA vaccine using a microarray analysis" 24 : 921-930, 2006

37 Ghislain Moussavou, "Baculovirus titration method based on MOI values for optimizing recombinant protein expression of the anti‐cancer vaccine candidate GA733‐Fc using Sf9 insect cells" 한국곤충학회 48 (48): 73-79, 2018

38 Håstein T, "Bacterial vaccines for fish – an update of the current situation worldwide" 121 : 55-74, 2005

39 Obembe OO, "Advances in plant molecular farming" 29 : 210-222, 2011

40 Plant KP, "Advances in fish vaccine delivery" 35 : 1256-1262, 2011

41 Lecocq-Xhonneux F, "A recombinant viral haemorrhagic septicaemia virus glycoprotein expressed in insect cells induces protective immunity in rainbow trout" 75 : 1579-1587, 1994

42 Gudding R, "A history of fish vaccination : science-based disease prevention in aquaculture" 35 : 1683-1688, 2013