Solution Plasma Process를 이용하여 합성한 Gelatin/Ag 나노입자 생체복합재료의 특성에 관한 연구 = A Study on the Characteristics of Gelatin/Ag Nanoparticles Biocomposite Synthesized by

다국어 초록 (Multilingual Abstract)

In recent, Solution Plasma Process has been developed to employ as a versatile process for nanoparticle formation. The biocomposite suspension of gelatin/Ag nanoparticles was prepared via the Ag nanoparticle formation in gelatin solution using solution plasma process in this study. The fabrication of 3D scaffold-type biocomposite using lyophilization for studying antimicrobial effects against various pathogenic microorganisms such as bacteria (Escherichia coli (-), Staphylococcus aureus (+)), yeast (Candida albicans), and fungi (Aspergillus parasiticus). The mixture of gelatin solution and Ag precursor (AgNO3) was first prepared and then it was discharged by high voltage generator under the controlled conditions to form the suspension of Ag nanoparticles in gelatin medium. The lyophilization was employed for fabricating the 3D scaffold-type biocomposite of gelatin/Ag from its suspension and the water-soluble property was improved by cross-linking using UV-irradiation (λ=254nm for 15min). And then, it was sterilized using ethylene oxide (EO) gas to apply in biomedical fields. The physical and chemical characteristics of both gelatin/Ag nanoparticles suspension and biocomposite were investigated using UV-Vis spectroscopy, TEM (Transmission Electron Microscope), EDS (Energy Dispersive Spectroscopy) and FE-SEM (Field Emission Scanning Electron Microscope) measurement. The results found that the formation of silver nanoparticles could be confirmed by the response of surface plasmon absorption in UV-Vis range and the good texture of micropores in biocomposite scaffold with Ag nanoparticle size of about 10~20 nm was observed in FE-SEM and TEM results. The good bactericidal effects of the synthesized biocomposite against various pathogenic microorganisms were observed confirmed by the results of clear zone size of Kirby-Bauer method, colony forming units test (CFU), minimum inhibitory concentration (MIC), and growth inhibition test (GIT). Gelatin/Ag nanoparticles biocomposite showed superior properties against micrococcus strain comparing with eumycetes strain. This biocomposite could be applicable for the anti-infection wound dressing.

목차 (Table of Contents)

• 목 차
• 제 1 장 서 론 --------------------------------------------- 1
• 제 2 장 이론적 배경
• 2.1 플라즈마 공학 ------------------------------------ 2
• 2.1.1 플라즈마의 정의 ------------------------------- 2
• 2.1.2 플라즈마의 성질 ------------------------------- 4
• 2.1.3 유체 플라즈마의 정의 및 특성 ----------------------- 6
• 2.2 플라즈마, 생명과학, 재료과학의 학문간 융합 ---------------- 7
• 2.2.1 플라즈마의 생명과학분야 접근 ---------------------- 7
• 2.2.2 플라즈마의 재료과학분야 접근 --------------------- 13
• 2.3 생체 재료 --------------------------------------------- 15
• 2.3.1 생체재료의 특성 ------------------------------------- 15
• 2.3.2 생체재료의 응용분야 ---------------------------------- 16
• 2.4 유체 플라즈마를 활용한 생체복합재료의 제조-------------------- 21
• 2.4.1 학문간 융합에 의한 연구 접근 -------------------------- 21
• 2.4.2 Ag 나노입자의 항균 메커니즘 --------------------------- 22
• 2.4.3 국내외 연구 동향 ----------------------------------- 23
• 제 3 장 실험방법
• 3.1 실험의 순서도 ------------------------------------------- 31
• 3.2 실험의 재료 및 방법 -------------------------------------- 31
• 3.3 유체 플라즈마 공정 --------------------------------------- 32
• 3.4 동결 건조 공정 ------------------------------------------ 33
• 3.5 후처리 공정 ---------------------------------------------- 34
• 3.6 Ag 나노입자 및 생체복합재료의 재료 특성 평가 ----------------- 35
• 3.7 생체복합재료의 생물학적 특성 평가 --------------------------- 36
• 제 4 장 결과 및 고찰
• 4.1 젤라틴/Ag 나노입자 복합재료의 물리적 특성 분석
• 4.1.1 젤라틴 기지재내 Ag 나노입자 합성-------------------39
• 4.1.2 젤라틴/Ag 나노입자 생체복합재료의 화학 및 광학적 특성 ------ 40
• 4.1.3 젤라틴/Ag 나노입자 생체복합재료의 전자 현미경 분석---- 43
• 4.2 젤라틴/Ag 나노입자 복합재료의 생물학적 특성 분석
• 4.2.1 억제대법 (Kirby-Bauer method)을 이용한 항균성 평가 ------ 50
• 4.2.2 Colony Forming Units를 통한 정량적 항균성 평가 --------- 53
• 4.2.3 Minimum Inhibitory Concentration을 통한 항균성 평가 ------ 59
• 4.3 젤라틴/Ag 나노입자 복합재료의 항균 효과 비교
• " 4.3.1 Ag 나노입자 크기에 따른 항균 효과 비교 ------------ 63
• 4.3.2 기지재 (Matrix)에 따른 항균 효과 비교 ------------ 64
• 4.3.3 Disc 크기 및 형태에 따른 항균 효과 비교-------- 65
• 제 5 장 결 론 -------------------------------------------- 67
• References ----------------------------------------------- 68