울릉도 자생 말오줌나무(Sambucus sieboldiana var. pendula) 잎으로부터 분리한 페놀성 물질이 LPS로 자극한 Raw 264.7 cell에서 나타내는 항염증 효과 = Anti-inflammatory Effect of Isolated Phenolics from Sambucus sieboldiana var. pendula Leaves on Raw 264.7 Cells with Stimulated Lipopolysaccharide

국문 초록 (Abstract)

본 연구에서는 말오줌나무잎 phenolics이 염증반응에 대해 나타내는 억제효과를 확인하였다. 항염증 효과를 확인하기 위해 Raw 264.7 대식세포에서 염증을 유발하는 LPS를 처리한 후 말오줌나무잎 phenolics이 염증 관련 인자 생성을 억제하는 효과를 ELISA, western blotting과 real-time PCR을 사용하여 측정한 결과, 말오줌나무잎 phenolics에 의해 염증인자인 NO 생성이 억제되었고, iNOS, COX-2 단백질 발현량이 억제되었으며, pro-inflammatory cytokine인 TNFα, IL1β, IL6, MCP1, PTGES2 등의 mRNA 발현이 유의적으로 억제되는 경향을 나타내었다. 따라서 말오줌나무잎 phenolics은 염증 발생에서 NF-κB의 신호전달에 의해 발현이 조절되는 iNOS, COX-2 protein의 발현억제와 염증반응에 관여하는 cytokine을 생산하는 TNFα, IL1β, IL6, MCP1, PTGES2 등의 mRNA 발현을 억제하여 높은 항염증효과를 나타내는 것을 확인할 수 있었다.

다국어 초록 (Multilingual Abstract)

Phenolic compounds are well known to be among the major bioactive compounds found in various plant sources. It has been reported that Sambucus sieboldiana, found on Ulleung Island in Korea, contains phenolic compounds, suggesting potential health benefits. Thus, in this study, we determined the inflammatory activity of Sambucus sieboldiana with Raw 264.7 mouse macrophage cells. Phenolic compounds isolated from Sambucus sieboldiana were exposed to LPS-stimulated macrophages to determine whether an anti-inflammatory effect existed. The inflammation-associated protein expression, activity, and gene expression levels were measured by means of ELISA, a western blot analysis, and qPCR. Phenolic compounds from Sambucus sieboldiana inhibited the expression of iNOS, COX-2, and their downstream products PGE2 and NO, which are part of the NF-kB signaling pathway. The mRNA gene expression levels of pro-inflammatory cytokines, in this case TNFα, IL1β, IL6, MCP1, and PTGES2, were significantly decreased. These results showed that phenolic compounds from Sambucus sieboldiana have an anti-inflammatory effect, suggesting that Sambucus sieboldiana can potentially be used as an anti-inflammatory treatment, such as therapeutic and/or cosmetic products for the skin, particularly for skin inflammation. However, further in vivo studies using phenolics from Sambucus sieboldiana are needed to support the current findings.