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다국어 초록 (Multilingual Abstract)

The optimal conditions for production of carboxymethylcellulase (CMCase) of Bacillus amyloliquefaciens DL-3 by a recombinant Escherichia coli JM109/DL-3were established at a flask scale using the response surface method (RSM). The optimal conditions of rice bran,tryptone, and initial pH of the medium for cell growth extracted by Design Expert Software were 66.1 g/L, 6.2 g/L, and 7.2, respectively, whereas those for production of CMCase were 58.0 g/L, 5.0 g/L, and 7.1. The analysis of variance (ANOVA) of results from central composite design (CCD) indicated that significant factor (“probe > F”less than 0.0500) for cell growth was rice bran, whereas those for production of CMCase were rice bran and initial pH of the medium. The optimal temperatures for cell growth and the production of CMCase by E. coli JM109/DL-3 were found to be 37oC. The optimal agitation speed and aeration rate of 7 L bioreactors for cell growth were 498 rpm and 1.4 vvm, whereas those for production of CMCase were 395 rpm and 1.1 vvm. The ANOVA of results indicated that the aeration rate was more significant factor (“probe > F” less than 0.0001) than the agitation speed for cell growth and production of CMCase. The optimal inner pressure for cell growth was 0.08 MPa,whereas that for the production of CMCase was 0.06 MPa.
The maximal production of CMCase by E. coli JM109/DL-3 under optimized conditions was 871.0 U/mL, which was 3.0 times higher than the initial production of CMCase before optimization.

참고문헌 (Reference)

1 Giavasis, I., "The effect of agitation and aeration on the synthesis and molecular weight of gellan in batch cultures of Sphingmonas paucimobilis" 38 : 101-108, 2006

2 Jecu, L., "Solid state fermentation of agricultural wastes for endogulcanse production" 11 : 1-5, 2000

3 Junker, B. H., "Scale-up methodologies for Escherichia coli and yeast fermentation process" 97 : 347-364, 2004

4 Khuri, A. I., "Response surfaces: Design and analysis" Marcel Dekker 1987

5 Lee, Y. J., "Purification and characterization of cellulase produced by Bacillus amyloliquefaciens DL-3 utilizing rice hull" 99 : 378-386, 2008

6 Kim, B. K., "Purification and characterization of carboxymethylcellulase isolated from a marine bacterium, Bacillus subtilis subsp. subtilis A-53" 44 : 411-416, 2009

7 Kang, S. W., "Production of cellulase and hemicellulases by Aspergillus niger KK2 from lignocellulosic biomass" 91 : 153-156, 2004

8 Krishna, C., "Production of bacterial cellulases by a solid state bioprocessing of banana wastes" 69 : 231-239, 1999

9 Yi, J. C., "Production and distribution of endoglucanase, cellobiohydrolase, and β- glucosidase components of the cellulolytic system of Volvariella volvacea, the edible straw mushroom" 65 : 553-559, 1999

10 Kalogeris, E., "Production and characterization of cellulolytic enzymes from the thermophilic fungus Thermoascus aurantiacus under solid state cultivation of agricultural waste" 38 : 1099-1104, 2003