A New Analytical Method for Erythromycin in Fish by Liquid Chromatography/Tandem Mass Spectrometry

다국어 초록 (Multilingual Abstract)

Erythromycin has been used to treat Streptococosis, Edwardsiellosis, Vibriosis, Bacterial enteritis in the cultured fish. In this study, a rapid and effective erythromycin analysis method with new sample treatment protocol and liquid chromatography/tandem mass spectrometry (LC/MS/MS) system for fish products was developed. For the erythromycin extraction from fish muscle, the solvent mixture composed of 0.2% meta-phosphoric acid and methanol (6:4) showed good recovery rate, and the optimum extraction solvent volume was 20 mL. Erythromycin detection using LC/MS/MS were carried out under electrospray ionization (ESI) positive condition and erythromycin mass value 576.2 and 157.9. And the detection limit of the established method was 0.005 mg/kg in fish products. The recovery rate of the developed method applied to the fish species were as following, olive flounder, 87.6±5.0%; black rockfish, 87.2±6.4%; eel, 85.2±4.8%; and rainbow trout, 86.0±6.2%. In the established method in this study, the correlation of coefficient values (R2) of erythromycin calibration curve (n=11) was 0.9998.

목차 (Table of Contents)

• Abstract
• Introduction
• Material and Methods
• Results and Discussion
• References

참고문헌 (Reference)

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