아연과 Phytic Acid 수준을 달리한 식이가 흰쥐의 단백질 대사에 미치는 영향 = Effect of Dietary Zinc and Phytic Acid Levels on Protein Metabolism in Rats

다국어 초록 (Multilingual Abstract)

In order to investigate the effect of dietary zinc and phytic acid levels on protein metabolism in rats, male rats of Sprague-Dawley strains weighing approximately $60\~74g$ were fed different diets which contained 0, 0.35 and $1.05\%$ phytic acid each at 3 levels of zinc(0, 30 and 1,500 ppm zinc) for 28 days. Result obtained in this experiment are summarized as follows; 1. Body weight gait food consumption food efficiency ratio and protein efficiency ratio were lower in the rats fed zinc deficient diet(0 ppm zinc) than in those consuming 30 or 1,500 ppm dietary zinc, and the additional effect of phytic acid were not observed in all of then 2. Liver weight was lower in the rats fed 30 ppm zinc diet than in those fed 0 or 1,500 ppm-zinc diet but kidney and spleen weights were lower in the rats fed zinc deficient diet than in those fed 30 or 1,500 ppm-zinc diet Among organs measured only the liver appeared to be influenced by dietary phytic acid: the more the dietary phytic acid, the more the weight of liver, 3. Fecal nitrogen was decreased in the rats fed zinc deficient diet compared with those fed 30 or 1,500 ppm dietary zinc. Urinary nitrogen was increased in the rats fed $1.05\%$ dietary phytic acid compared with those fed 0.35 or $0\%$ dietary phytic acid Nitrogen retention of rat was influenced by neither dietary zinc nor phytic acid. 4. Urea nitrogen was decreased with increasing dietary zinc levels, and creatinine and uric acid levels were increased with increasing dietary zinc concentration or with additional quantity of phytic acid. Uric acid appeared to be influenced by zinc x phytic acid interaction; especially, the presence of phytic acid in the 30 ppm-zinc diet had significant effect on uric acid content. 5. Hemoglobin concentrations and hematocrit ratio were higher in the rats fed 30 ppm dietary zinc than in those fed 0 or 1,500 ppm-zinc diet Serum zinc concentration was increased with increasing dietary zinc levels. The content of total protein albumin and BUN and the ratio of albumin to globulin in serum, and protein content in liver were influenced by neither dietary zinc nor phytic acid.

참고문헌 (Reference)

1 "skeletal and allometric changes due to zinc deficiency in the baby pig" 278-286, 1968

2 "Zinc toxicity" 51 : 225-227, 1990

3 "Zinc requirement and balance studies with the rat" 70 : 53-57, 1960

4 "Zinc in the nutrition of the rat" 107 : 146-156, 1934

5 "Zinc deficiency syndrome in the young lamb" 82 : 41-50, 1964

6 "Zinc deficiency in the weanling rat Effect on liver composition" 1152-1158, 1976

7 "Zinc and nitrogen balance in adolescent females fed varying levels of zinc and soy protein" 31 : 112-116, 1978

8 "Wiley,New York" 1957

9 "Wet ashing apparatus to prepare biological materials for atomic absorption spectrophotometry" 20 : 859-861, 1971

10 "Trace elements in Human and Animal Nutrition" Academic Press Inc New York 1977p196-242

11 "The effects of zinc deficiency on pancreatic carboxypeptidase activity and protein digestion and absorption in the rat" 102 : 712-718, 1967

12 "The effect of interactions between dietary egg white protein and zinc body weight bone growth and tissue trace metals in the 30-day-old rat" 1307-1320, 1983

13 "Studies on the phytate:zinc molar contents in diets as determinant of Zn availability to young rats" 591-603 1979

14 "Studies on growth copper metabolism and iron metabolism of rats fed high levels of zinc" 233-242, 1960

15 "Severe zinc deficiency in male and female rats" 95 : 8-18, 1969

16 "Second and final report" 211 1958

17 "Report of the AIN Ad Hoc Committee on standards for nutritional studies" 107 : 1340-1378, 1977

18 "Relation of zinc metallo-enzyme activities to zinc concentrations in tissues" 215 : 430-437, 1970

19 "Rapid determination of urea nitrogen in serum or plasma without deproteinization" 33 : 361-365, 1967

20 "Procedure for serum protein determinations In standard methods of clinical chemistry" Academic Press Inc 7 1972

21 "Nitrogen retention during late gestation in the rat in response to marginal zinc intake" 239 : 1980

22 "Influence of phytate and low dietary calcium on calcium phosphate and zinc metabolism by growing rats" 145-155, 1993

23 "Experimental zinc deficiency in guinea-pigs" 55 : 613-620, 1986

24 "Effects of zinc deficiency on ethanol metabolism and alcohol and aldehyde dehydrogenase activities" 104 : 610-617, 1984

25 "Effects of phytic acid on zinc availability and parakeratosis in swine" 21 : 57-61, 1962

26 "Effects of isolated parameters of the composition of skeletal muscle liver and bone during growth in rats" 610-617, 1986

27 "Effect of zinc deficiency on urinary excretion of nitrogenous compounds and liver amino acid-catabolizing enzymes in rats" 105 : 26-31, 1975

28 "Effect of zinc deficiency on protein synthesis in brain and liver of suckling rats" 107 : 1082-1093, 1977

29 "Effect of zinc deficiency on plasma proteins of young Japanese quail" 86 : 89-92, 1965

30 "Effect of zinc deficiency on growth hematological values and tissues zinc content in rats" 281-290, 1982

31 "Effect of zinc deficiency on enzyme activities lipids and nitrogen compounds contents in serum of rats" 5-13, 1983

32 "Effect of zinc deficiency on appetite and plasma amino acid concentrations in the rat" 45 : 127-136, 1981

33 "Effect of soybean fiver and phytate on serum zinc response" 9 : 127-132, 1989

34 "Effect of source and level of dietary protein on the toxicity of zinc to the rat" 74 : 51-57, 1961

35 "Effect of protein level and protein source on zinc absorption in humans" 119 : 48-53, 1989

36 "Effect of phytic acid on zinc availability" 103 : 304-305, 1960

37 "Effect of phytic acid on the availability of zinc in amino acid and casein diets fed to chicks" 84 : 145-148, 1964

38 "Effect of excess dietary zinc on iron and copper in the rat" 70 : 514-520, 1960

39 "Effect of different dietary zinc and protein levels on lipid metabolism" 22 : 9-22, 1989

40 "Effect of dietary zinc and copper interrelationships on blood parameters of the rat" 24 : 808-811, 1976

41 "Effect of dietary copper manganese and zinc on nitrogen equilibrium and mineral distribution subsequent to trauma in mature rats" 1421-1428, 1976

42 "Determination of serum albumin In standard methods of clinical chemistry" Academic Press Inc 7 1972

43 "A modified carbonate-phosphotungstate method for the determination of uric and comparison with the spectrophotometric urease method" 28 : 152-160, 1957

44 "A comparison of three methods" 222-238 1968