흰쥐에서 혈청 (血淸) Fructose - 1 , 6 - Diphosphatase 측정에 의한 문맥주위 (門脈周圍) 및 간소엽중심부 (肝小葉中心部) 괴사의 (壞死) 판별 = Differentiation of Periportal and Pericentral Necrosis by Measurement of Serum Fructose - 1 , 6 - diphosphatese Activities in Rats흰쥐에서 혈청 (血淸) Fructose - 1 , 6 - Diphosphatase 측정에 의한 문맥주위 (門脈周圍) 및 간소엽중심부 (肝小葉中心部) 괴사의 (壞死) 판별

다국어 초록 (Multilingual Abstract)

N/A

다국어 초록 (Multilingual Abstract)

In order to test the hypothesis that periportal(acinar zone 1) and pericentral(acinar zone 3) necrosis can be differentiated by measurement of serum fructose-l, 6-diphosphatase (FDP) which has been reported to be predominanely distributed in the periportal zone of the acinus, the enzyme was assayed in serum during periportal necrosis induced by allyl alcohol(0.62m mole/kg, dissolved in 0.9% NaCl Itraperitoreally) and during pericentral necrosis by bromobenzene(3.80m mole/kg, dissolved in corn oil, intraperitoneally), and the activities were compared in two conditions. Serum FDP activities in normal saline control, allyl alcohol-treated normal histologic finding, minimal, moderate and severe periportal necrosis groups were 0.005+-0.018, 0.048+-0.018,0.090+-0.009,0.113+-0.029 and 0.140+-0.010 unit/ml, respectively. The levels in moderate and severe periportal recros group were significantly(p<p.01) elevated in comparison with those of normal control. Serum FDP activities in normal corn oil control, bromobenzene treated normal histologic finding, minimal, moderate and severe pericentral necrosis groups were 0.056+-0.014, 0.048+-0.014. 0.059+-0.021, 0.078+-0.021 and 0.124+-0.12 unit/ml, respectively, and it showed that the levels in minimal and moderate necrosis groups were nearly the same as those in normal control, and only those in group of severe necrosis showed significant (p<0.0l) elevation. The ALT and FDP activities between two groups have significantly correlated (r=0.665, r = 0.724), but the regression equation that is y=0.0085 x+0.069 or y=0.01 x +0.04 respectively, was significantly different. These results indicated that at least experimental necrosis of the periportal and perice- ntra! Zones in the rats can be differentiated by measurement of serum FDP. And it was suggested that simultaneous measurements of either serum beta-hydroxybutyric dehydrogenase or pyruvate kinase which was reported to be predominantly distr ibuted at the pericentral zone would help to differentiate more precisely.