표고 및 구름버섯으로부터 Mn - peroxidase 와 Laccase 의 생산 = Production of Mn - peroxidase and Laccase from Lentinus edodes and Coriolus versicolor

다국어 초록 (Multilingual Abstract)

This study was undertaken to investigate the characteristic, and the productivities of lignin olytic enzymes: lactase (Lac) and Mn-dependent peroxidase(MnP) from Coriolus versicolor and Lentinus edodes respectively. Enzymes were isolated from cultural filterates and purified according to the standard methods. These enzymes showed one band in SDS-PAGE and their molecular weights were found 62,000 and 45,000 dalton respectively. Polyclonal antibodies against Lac and MnP were raised against mouse, In the ELISA (enzyne-lurked immunosorbent assay). Lac and MnP-antiserum produced a strong positive reaction with Lac and MnP antigen (S_(405)=2.50 and 3.53 respectively). The sera to negative (S/N)ratio way determined by the dividing the mean absorbance of antibodies by the corresponding diluted samples from normal mouse serum. The sera produced showed 2 times more positive reaction in S/N ratio than negative sera.