흰민들레, 고들빼기, 씀바귀 추출물의 암세포 증식 억제 효과 = Induction of Apoptosis in Human Cancer Cells with Extracts of Taraxacum coreanum, Youngia sonchifolia and Ixeris dentate

다국어 초록 (Multilingual Abstract)

This research examined the effects of Compositae extract on the inhibition of proliferation and apoptosis in human breast and human gastric cancer cells. Compositae extracts which is used in the experiment are Taraxacum coreanum(TC), Youngia sonchifolia(YS) and Ixeris dentata(ID). The proliferation of SK-BR-3, MDA-MB-231 and AGS cells were investigated by MTT assay. ID and YS extracts inhibited proliferation of SK-BR-3, MDA-MB-231 and AGS cells in a dose-dependent manner, but TC have barely affected. In addition, the most effective extract was ID. To assess the apoptosis of ID extract, the nuclei of human cancer cells were stained with DAPI solution respectively. Chromatin condensation, indicated apoptosis, was increased in a dose-dependent manner. We investigated change of ID extract-induced apoptosis proteins on human cancer cells by western blot analysis. The level of Bcl-2 decreased, whereas the level of Bax, cleaved-PARP increased in dose-dependent manner compared with non-treatment. Also Bax/Bcl-2 ratio, which is used in clinical indicator of apoptosis, was increased at ID extract treatment group compared with non-treatment. Moreover the Bax/Bcl-2 ratio of MDA-MB-231 cell was significantly increased as against SK-BR-3, AGS cells. These results indicated that ID extract have anti-proliferation effect better than YS or TC, and induced apoptosis in human breast cancer MDA-MB-231 cell better than human breast cancer SK-BR-3 cell, human gastric cancer. Even if further research is needed, ID can be developed as a chemopreventive or therapeutic agent of breast cancer.

참고문헌 (Reference)

1 이현화, "흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교" 한국약용작물학회 16 (16): 79-85, 2008

2 임도연, "흰민들레 에탄올 추출물 및 분획물의 항산화 및 항균활성과 Tyrosinase 저해활성" 한국약용작물학회 19 (19): 238-245, 2011

3 김지은, "유자와 탱자 과피 에탄올 추출물의 MCF-7 유방암 세포에 대한 항암 활성" 한국생명과학회 18 (18): 1435-1441, 2008

4 김명조, "씀바귀의 항돌연변이성 및 암세포 성장억제효과" 한국약용작물학회 10 (10): 11-143, 2002

5 이은, "씀바귀 추출물이 LPS투여 흰쥐 및 Raw 264.7세포에서 전염증성 cytokines 생성에 미치는 영향" 한국자원식물학회 24 (24): 604-612, 2011

6 김명조, "씀바귀 뿌리 추출물의 항상화성, 항돌연변이원성 및 항암활성 효과" 한국약용작물학회 10 (10): 222-229, 2002

7 윤보라, "쓴메밀 및 단메밀 에탄올 추출물의 항산화 및 지방세포 분화억제 효과" 한국식품저장유통학회 19 (19): 123-130, 2012

8 이현영, "상염색체 우성 다낭신에서 세포자멸사에 대한 연구" 대한신장학회 24 (24): 366-374, 2005

9 안인정, "국화과 추출물의 암세포 증식 억제 효과" 한국식품영양과학회 41 (41): 584-590, 2012

10 이미경, "국화과 약용 식물의 면역증진활성 검색" 한국약용작물학회 10 (10): 10-57, 2002

11 우정향, "국화과 식물 중 꽃 에탄올추출물의 항산화효과" 한국식품영양과학회 39 (39): 159-164, 2010

12 김미정, "고들빼기의 성분분석과 항산화효과" 한국식품위생안전성학회 25 (25): 354-359, 2010

13 천상욱, "고들빼기 부위별 메탄올 추출물의 폴리페놀 함량 및 항산화성 연구" 한국작물학회 58 (58): 20-27, 2013

14 Lee M. H., "The aerial part of Taraxacum coreanum extract has an anti-inflammatory effect on peritoneal macrophages in vitro and increases survival in a mouse model of septic shock" 146 : 1-8, 2013

15 Schütz K., "Taraxacum—a review on its phytochemical and pharmacological profile" 107 : 313-323, 2006

16 Dorssers, L. C. J., "Tamoxifen resistance in breast cancer" 61 : 1721-1733, 2001

17 Kang J.O., "Special Editions : Radiation Therapy of Breast Cancer" 21 : 37-42, 2005

18 Moon Y. O., "Regional variation in accessing regional hospitals for cancer patients" 28 : 152-161, 2006

19 Zhang L., "Proteomic analysis reveals molecular biological details in varioliform gastritis without Helicobacter pylori infection" 16 : 3664-3673, 2010

20 Yang J., "Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked" 275 : 1129-1132, 1997

21 Park S.S., "Original Articles; Studies on the Constituents and Their Biological Activities of Ixeris Sonchifolia Hance (1)" 10 : 241-252, 1977

22 Chung H. S., "Nitric oxide and tumor necrosis factor-α production by Ixeris dentata in mouse peritoneal macrophages" 82 : 217-222, 2002

23 Cha M. R., "New guaiane sesquiterpene lactones from Ixeris dentata" 77 : 380-382, 2011

24 Doonan F., "Morphological assessment of apoptosis" 44 : 200-204, 2008

25 Pecorino, L., "Molecular biology of cancer: mechanisms, targets, and therapeutics" Oxford university press 157-182, 2012

26 Park, Eun-Kyung, "Lactic Acid Bacterial Fermentation Increases the Antiallergic Effects ofIxeris dentata" 한국미생물·생명공학회 18 (18): 308-313, 2008

27 Nam S.H., "Isolation of cytotoxic substances from Chrysanthemum boreale M" 38 : 273-277, 1995

28 Kim, S.H., "Inhibitory effects of Ixeris dentata on the mutagenicity of aflatoxin B1, N-methyl-N'-nitro-N-nitrosoguanidine and the growth of MG-63 human osteosarcoma cells" 24 : 305-312, 1995

29 Oliver, F. J., "Importance of poly (ADP-ribose) polymerase and its cleavage in apoptosis Lesson from an uncleavable mutant" 273 : 33533-33539, 1998

30 Shin S.C., "Exploitation of the biologically active components in Youngia sonchifolia Max" 36 : 134-137, 1993

31 Bae S. J., "Effects of Godulbaegi (Ixeris sonchifolia H.) diet on enzyme activities of CCl4 induced hepatotoxicity in rats" 30 : 19-24, 1997

32 Lim S.S., "Effect of Aster scaber and Ixeris dentata on contractility and vasodilation of cardiovascula and endothelial cell in hyperlipidemic rat" 26 : 300-307, 1997

33 Roth, A., "Docetaxel-based chemotherapy in the treatment of gastric cancer" 14 : ii41-ii44, 2003

34 안은미, "Cytotoxic and ACAT-inhibitory Sesquiterpene Lactones from the Root of Ixeris dentata forma albiflora" 대한약학회 29 (29): 937-941, 2006

35 Kang, M.J., "Current trends of research and biological activities of dandelion" 6 : 60-67, 2001

36 Lee, T.B., "Coloured Flora of Korea"

37 Rivera, F., "Chemotherapy of advanced gastric cancer" 33 : 315-324, 2007

38 Kothakota, S., "Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis" 278 : 294-298, 1997

39 Oltval Z. N., "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death" 74 : 609-619, 1993

40 Reed J.C., "Bcl-2 and the regulation of programmed cell death" 124 : 1-6, 1994

41 Heo B. G., "Antiproliferative activity of Korean wild vegetables on different human tumor cell lines" 64 : 257-263, 2009