Simple Sequence Repeat (SSR) 마커를 이용한 장미 품종 식별

국문 초록 (Abstract)

본 연구는 국내외에서 육성 및 수집된 장미품종에 대하여 SSR 마커를 이용하여 품종식별 연구를 수행하였다. 장미 품종식별에 적합한 SSR 마커를 선정하기 위하여 112개의 SSR 마커를 12개의 주요품종을 대상으로 분석하였다. 최종 선발된 22개의 SSR 마커를 대상으로 69품종을 이용하여 분석하였을 때 대립유전자의 수는 2～10개의 분포를 나타내었으며 총 114개의 대립유전자가 분석되었다. PIC 값은 0.211～ 0.813의 범위에 속하였으며 평균값은 0.621로 나타났다. SSR 마커를 이용하여 작성된 장미 69품종의 품종간 유전적 거리는 0.41～0.87의 범위로 나타났고, 공시 품종 모두 SSR 마커의 유전자형에 의해 구분되었다. 본 연구결과는 장미 품종의 식별과 유전적 다양성 연구를 위한 기초 자료로 유용하게 활용될 수 있을 것으로 기대된다.

다국어 초록 (Multilingual Abstract)

The objective of this study was to evaluate the suitability of simple sequence repeat markers for variety identification in 69 rose (Rosa x hybrida) varieties. A set of 112 SSR primer pairs was evaluated and 43 primer pairs showed polymorphism in 12 varieties. Twenty-two primer pairs out of 43 primer pairs showed high levels of polymorphism and reproducibility. The genetic relationship of 69 varieties was analyzed based on the marker genotypes of 22 SSRs. A total of 114 polymorphic amplified fragments were obtained by using 22 SSR markers. Two to ten SSR alleles were detected for each locus with an average of 5.18 alleles per locus. Average polymorphism information content (PIC) was 0.621, ranging from 0.211 to 0.813. A total of 114 marker loci were used to calculate Jaccard’s distance coefficients for cluster analysis using unweighted pair-group method with arithmetical average (UPGMA). Cluster analysis of genetic diversity revealed that these SSR marker sets identified each genotypes of 69 rose varieties. These SSR markers may be used for wide range of practical application in variety identification of rose.

참고문헌 (Reference)

1 Nishitani C, "Tri-/Hexanucleotide Microsatellite Markers in Peach Derived from Enriched Genomic Libraries and Their Application in Rosaceae" 57 : 289-296, 2007

2 Mantel N., "The detection of disease clustering and a generalized regression approach" 27 : 209-220, 1967

3 Gudin S., "Rose: genetics and breeding" 17 : 159-189, 2000

4 Park YH, "Rose (Rosa hybrida L.) EST-derived microsatellite markers and their transferability to strawberry (Fragaria spp.)" 125 : 733-739, 2010

5 Anderson JA, "Optimizing parental selection for genetic linkage maps" 36 : 181-186, 1993

6 Sneath PHA, "Numerical taxonomy: The principles and practice of numerical classification" W. H. Freeman 1973

7 Rohlf FJ., "Numerical Taxonomy and Multivariate analysis system-version 2.10b" Applied Biostatistics Inc 2000

8 권용삼, "Microsatellite 마커를 이용한 한국 보리 품종의 유전적 다양성" 한국육종학회 43 (43): 322-329, 2011

9 Esselink GD, "Identification of cut rose (Rosa hybrida) and rootstock varieties using robust sequence tagged microsatellite site markers" 106 : 277-286, 2003

10 Hibrand-Saint Oyant L, "Genetic linkage maps of rose constructed with new microsatellite markers and locating QTL controlling flowering traits" 4 : 11-23, 2008

11 Shiferaw E, "Exploring the genetic diversity of Ethiopian grass pea (Lathyrus sativus L.) using EST-SSR markers" 30 : 789-797, 2011

12 Leal AA, "Efficiency of RAPD versus SSR markers for determining genetic diversity among popcorn lines" 9 : 9-18, 2010

13 Kimura T, "Development of microsatellite markers in rose" 6 : 810-812, 2006

14 Weising K, "DNA fingerprinting in plants: principle, methods, and applications" CRC Press 39-, 2005

15 Reid A, "Construction of an integrated microsatellite and key morphological characteristic database of potato varieties on the EU common catalogue" 182 : 239-249, 2011

16 Yan Z, "Construction of an integrated map of rose with AFLP, SSR, PK, RGA, RFLP, SCAR and morphological markers" 110 : 766-777, 2005

17 Bredemeijer GMM, "Construction and testing of a microsatellite database containing more than 500 tomato varieties" 105 : 1019-1026, 2002

18 UPOV-BMT, "BMT/9/12 Analysis of a Database of DNA Profiles of 734 hybrid Tea Rose (Rosa Hybrida) Varieties"

19 UPOV-BMT, "BMT/12/02 Reports on Developments in UPOV Concerning Biochemical and Molecular Techniques"

20 권용삼, "Assessment of Genetic Variation among Commercial Tomato (Solanum lycopersicum L.) Varieties Using SSR Markers and Morphological Characteristics" 한국유전학회 31 (31): 1-10, 2009

21 Gi-Jun Kim, "Application of UPOV Data for the Analysis of Genetic Variation in Rose Cultivars" 한국원예학회 29 (29): 240-246, 2011