중복기생균 Ampelomyces quisqualis 94013의 배양적 특성 = Cultural Characteristics of a Hyperparasite, Ampelomyces quisqualis 94013

다국어 초록 (Multilingual Abstract)

Ampelomyces quisqualis 94013 (AQ94013), a hyperparasite, was selected as an effective biological control agent against cucumber powdery mildew. Optimal temperature for mycelial growth of AQ94013 was 26oC,
and the optimal pH was 6.5. Conidia of AQ94013 were more produced on potato dextrose agar (PDA) in darkness than under alternating cycles of 12 hr fluorescent light and 12 hr darkness. Temperature range for
spore germination of the fungus was 10~35oC, and optimal temperature was 20oC. Conidial germination of the fungus began 8 hr after incubation at 24oC. Germination rate of conidia at concentration of 5 × 105 spores/ml and 5 × 106 spores/ml was higher than at concentration of 5 × 107 spores/ml. The best source of carbon and
nitrogen for mycelial growth of the fungus were dextrin and neopeptone, respectively.

국문 초록 (Abstract)

Ampelomyces quisqualis 94013는 중복기생균으로 흰가루병 생물적 방제용 우수균주로 선발하여, 이 균의 효율적 이용을 위한 배양적 특성을 조사하였다. A. quisqualis 94013의 균사생육 적온은 26oC이였으며, 균사생육 최적pH는 6.5였다. 감자한천배지(PDA)배양시 광조건(형광등 12시간주기)보다는 암조건에서 더 많은 포자를 형성하였다. 이 균의 포자발아 온도범위는 10~35oC이고, 최적온도는 20oC이였다. 포자발아는 24oC에서 8시간 배양 후 시작되었으며, 포자발아율은 포자현탁액의 농도가 5×107/ml보다 5×105/ml나 5×106/ml에서 높았다. 이 균은 탄소원으로는 dextrin을, 질소원으로는 neopepton을 가장 잘 이용하였다.

참고문헌 (Reference)

1 "한국식물병명목록" 779 -, 2004

2 "Water agarose medium forstudying factors affecting germination of conidia ofAmpelomyces quisqualis" 101 : 422-424, 1997

3 "Use of hyperparasites in biological controlof biotrophic plant pathogens Microbiology of thePhyllosphere" Cambridge Univ Press 333-347, 1986

4 "The relationship between thedevelopment of haustoria of Erysiphe graminis and the energystatus of leaves In Current Research in Photosynthesis" KluwerAcademic Publishers 223-226, 1990

5 "Studies on the life-cycle of cucurbit powderymildew fungus Sphaerotheca fuliginea" 1-106, 1989

6 "Powderymildews recent Advances toward integrated control" 89-126, 1998

7 "Parasitismus vonAmpelomyces quisqualis auf Echten Mehltaupilzen an Gurtenund anderen Gemusearten" 129-142, 1979

8 "New approaches in the study of thegenus Ampelomyces Abstracts of the 3rd EFPP Conference" 83-, 1994

9 "Modern fungicides and antifungal compounds" 1996

10 "Isolation of hyperparasiticfungi to powdery mildews and selection of superior isolatesfor biocontrol of cucumber powdery mildew" 141-151, 1994

11 "Efficient Production ofphialoconidia of Verticillium lecanii for biocontrol ofcucumber powdery mildew Sphaerotheca fuliginica" Verhaar 101-103, 1993

12 "Distributions ofsensitivities to three sterol demethylation inhibitor fungicidesamong populations of Uncinula necator sensitive and resistantto triadimefon" 87 : 784-87 791, 1997

13 "Current status insensitivity of Sphaerotheca fuliginea to DMI in Kanto-TosanDistrict" 69-75, 1994

14 "Cultivation of Ampelomyces quisqualis and interference offungicides in its growth in vitro" 21 : 26-29, 1996

15 "Ampelomyces quisqualis for biological and integrated controlof powdery mildews in Israel" 285-295, 1989

16 "A Monograph of the Erysiphales" Borntraeger Publisher 700-, 1987

17 "1986.Host range and geographical distribution ofthe powdery mildew" tokyo : press