The growth stimulation of Lactobacillus bulgaricus ATCC 11842 by sodium formate, and the mixed culture of Lactobacillus bulgaricus and Streptococcus thermophilus was examined at 45℃ for 10-48 hrs. respectively. The periodical change of lactic acid p...
The growth stimulation of Lactobacillus bulgaricus ATCC 11842 by sodium formate, and the mixed culture of Lactobacillus bulgaricus and Streptococcus thermophilus was examined at 45℃ for 10-48 hrs. respectively. The periodical change of lactic acid production and viable count were analyzed.
The results obtained are as follows:
1) When the mixed culture of L. bulgaricus and Str. thermophilus in 11% skim milk media was incubated by stationary culture, the lactic acid production was stimulated after 36 hrs. but was not stimulated in case of shaking culture at 140 rpm.
2) The lactic acid production of L. bulgaricus ATCC 11842 was more increased in 11% skim milk media sterilized at 121℃ for 15 minutes than in 11% skim media tyndallized for 2 days.
3) The lactic acid production and viable count of L. bulgaricus ATCC 11842 in 11% skim milk media added 65 ppm sodium formate were increased considerably.
4) The maximum lactic acid content of L. bulgaricus ATCC 11842 in 11% skim milk media added sodium formate was 0.55% at 100-110 ppm sodium formate.
And the lactic acid production was completely inhibited at 200 ppm sodium formate.