미세조류 Dunaliella bardawil을 이용한 β-carotene 생산조건에 관한 연구 = Production of β-carotene from high density algal culture of Dunaliella bardawil

다국어 초록 (Multilingual Abstract)

Low-growth rates favor β-carotene accumulation under conditions of stress, light intensity and wave length, oxygen free radicals, and high salinity. It was found from shake flask experiments that high density of algal cultures can be increased by the following environmental condition : white fluorescent light compared with blue and red light, addition five times concentrated medium, mixture of 104㎖/day CO_2 gas and 250mM sodium bicarbonate, and pH 8.0. The periodic feeding of 7mM nitrate ion under the nitrogen limitation condition enhanced the cell concentration up to 9 × 10 exp (6) cells/㎖ compared with 1.5 × 10 exp (6) cells/㎖ of the control culture. An photobioreactor system was successfully for algal growth and resulted in max cell density of 4.2 × 10 exp (6) cells/㎖ in comparison with that of batch flask culture(generally max cell density of batch flask culture is about 3.0 × 10 exp (6) cells/㎖). The following induction conditions such as change of salinity(NaCl), oxygen free radicals1 (H_2O_2), and blue light favored the massive accumulation of β -carotene. The change of culture conditions from a phase of favorable high cell density to that of optimum induction factors resulted in increased productivity.