시설재배 부추(Allium tuberosum R.)에 대한 살균제 Tebuconazole의 잔류특성 연구 = Residual Characteristic of Fungicide Tebuconazole in Chinese chives (Allium tuberosum R.) under Greenhouse Condition

국문 초록 (Abstract)

본 연구는 시설재배 부추를 대상으로 tebuconazole 수화제(25%)를 살포하여 경과일수에 따른 잔류특성 조사와 위험평가를 통해 안전사용기준설정을 위한 기초자료로 활용하고자 수행되었다. 포장시험은 공시 약제를 2,000 배로 희석하여 수확 예정일을 기준으로 수확 당일, 수확 7일전, 수확 14일전 및 21일전에 맞추어 2-3회 살포하였다.
수거한 시료를 QuEChERS시험법으로 전처리한 후 LC-MS/MS를 이용하여 분석하였고, 분석방법에 의한 정량한계는 0.01 mg/kg, 검량선의 직선성(r2)은 0.9996, 저농도(10 μg/kg) 및 고농도(100 μg/kg)에 대한 평균 회수율은 95.7- 102.1% (C.V. 2.8-4.0%)이었다. 잔류량 분석결과 수확 21-14일전 2회 살포시 3.29 mg/kg (최대 잔류량 3.62 mg/kg), 수확 21-14-7일전 3회 살포 시 7.22 mg/kg (최대 잔류량 7.39 mg/kg), 수확 14-7-0일전 3회 살포시 9.71 mg/kg (최대잔류량 9.89 mg/kg)으로 잔류하였다. 위험평가 결과 tebuconazole의 최대잔류량(9.89 mg/kg)으로부터 산출한 ADI 대비 식이섭취율(%ADI)의 값은 1.88%으로 나타났고, 이론적 일일최대섭취량인 TMDI는 29.0%로 산출되어 부추에 대한 tebuconazole의 노출은 상당히 안전한 수준인 것으로 확인되었다.

다국어 초록 (Multilingual Abstract)

This study was carried out to investigate the residual characteristic of fungicide tebuconazole in Chinese chives under greenhouse condition. The fungicide tebuconazole (25% wettable powder) diluted by 2,000 times was applied three times with one-week interval and then samples were collected at the date of harvest. The fungicide tebuconazole was extracted with QuEChERS method and residue was analyzed by LCMS/ MS. Method limit of quantitation(MLOQ) in tebuconazole was 0.01 mg/kg (limit detected 0.03 ng) and mean recovery were 95.7-102.1% (low level-high level). The matrix matched calibration curves for tebuconazole in Chinese chives yielded good linearity (r2>0.9996). The fungicide tebuconazole contents ranged were 3.06- 3.62 mg/kg (before harvest 21-14 days), 7.05-7.39 mg/kg (before harvest 21-14-7 days), and 9.51-9.89 mg/kg (before harvest 14-7-0 days). The amounts of tebuconazole residues in Chinese chives was higher as spraying closer to harvest day. The theoretical maximum daily intake (TMDI) of diet was 29.0% (% acceptable dietary intake, tebuconazole acceptable dietary intake was 0.03 mg/kg b.w./day). Based on the results of this study, It would contribute on the safe use for the pesticides and management for the safe agricultural products.

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