Effects of Culture Type and Inoculation Quantity in Bioreactor on Production of Potato Plantlets

다국어 초록 (Multilingual Abstract)

Potato (Solanum tuberosum Dejima) plantlets were investigated on culture type and initialquantity of inoculation in bioreactor and survival rate by hydroponics for mas production. Node stems (1 toSkoog (MS) medium with sucrose 30 g L-1. When plantlets (80-node inoculation) were raised in 10 L bal-loon type bubble (BB) bioreactor, the healthiest growth of plantlets was obtained from explants cultured inebb & flow culture with medium supplied periodically 12 times per day. The suitable inoculation quantity of20 L BB bioreactor was 120 pieces of stem segments (mean 2.2 g fresh weight) in ebb & flow culture. Num-ber of nodal shot was eight on the average. In controlled culture rom, survival rate of plantlets at 7 daysolid medium culture. The highest survival rate of the stem cutting plantlets were in nutrient solutionadjusted to EC 1.4 dSm-1. Stem cutting plantlets through one culture could be obtained 670~900, whenplantlets were grown in ebb & flow culture during 3 weeks using a 20 L bioreactor with initial 120 pieces ofnodal segments. It is posible to do mas production of seedlings cultured in bioreactor and hydroponics.

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