다양한 식물추출물을 활용한 in vitro 반추위 내 메탄저감 연구

다국어 초록 (Multilingual Abstract)

The present study was conducted to investigate effect of various plant extracts on enteric rumen methane emission. A possible interaction of used plant extracts was also examined using a fractional factorial experimental design. Ethanol extracts of lemon peel, orange peel, garlic by-product, spent coffee ground and lotus leaf were employed as plant extracts sources. Antioxidant activity and polyphenol content of extracts were measured. In vitro rumen simulated fermentation was performed to evaluate methane emission activity. Rumen fluid obtained from Holstein steer was strained through 8 layers cheese cloth and then mixed with McDougall solution in ratio of 1 to 4 to prepare rumen inoculum. Total mixed ration (TMR) was used as basal diet for fermentation and each extracts were included according to experimental design. Plackett burman design was employed as experimental design. In vitro rumen fermentation was performed for 24 hours at 39℃. Gas production, ruminal pH, methane and hydrogen gas, ammonia nitrogen and volatile fatty acid profile analysis were followed. At single extract effect evaluation, extracts of garlic by-product and spent coffee showed effective in vitro rumen methane mitigation without negative impact on rumen fermentation. In the interaction evaluation for used plant extracts, significant interaction between garlic by-product and spent coffee was detected in terms of in vitro rumen methane mitigation without suppression of rumen fermentation. As a conclusion, garlic by-product and spent coffee ground extracts were selected from other extracts as a possible environment friend plant extract for reduction of enteric rumen methane production.

목차 (Table of Contents)

• 1. 서론 1
• 2. 재료 및 방법 5
• 2.1. 공시재료 5
• 2.2. 식물추출물의 생리활성 5
• 2.2.1. 항산화활성 측정 5
• 2.2.2. 폴리페놀 측정방법 6
• 2.3. 반추위 in vitro 발효성상 6
• 2.3.1. 시험설계 6
• 2.3.2. 기초사료 8
• 2.3.3. 공시동물 사양관리 및 반추위액 채취 9
• 2.3.3.1. 공시동물 사양관리 9
• 2.3.4. 분석항목 9
• 2.3.4.1. pH 9
• 2.3.4.2. 암모니아태 질소(ammonia nitrogen, NH3-N) 10
• 2.3.4.3. 총 가스 생성량 10
• 2.3.4.4. 휘발성 지방산(volatile fatty acid, VFA) 10
• 3. 결과 및 고찰 11
• 3.1. 추출물 내 생리활성 역가 11
• 3.2. 반추위 in vitro 발효성상 13
• 3.2.1. 반추위 pH 15
• 3.2.2. 암모니아태 질소 20
• 3.2.3. 총 가스 생성량 25
• 3.2.4. 수소 생성량 30
• 3.2.5. 메탄 생성량 35
• 3.2.6. 총 휘발성 지방산 40
• 3.2.7. Acetate 45
• 3.2.8. Propionate 50
• 3.2.9. Butyrate 55
• 3.2.10. Valerate 60
• 3.3. 고찰 65
• 4. 결론 67
• 5. 인용문헌 68