Although the polydimthylsiloxane (PDMS) microfluidic devices are frequently utilized in microfluidic applications, these devices have several problems such as short-term modified surface condition, swelling in the presence of organic solvents and defo...
Although the polydimthylsiloxane (PDMS) microfluidic devices are frequently utilized in microfluidic applications, these devices have several problems such as short-term modified surface condition, swelling in the presence of organic solvents and deformation under high pressure. To solve these problems, Norland Optical Adhesive (NOA) has been presented as alternative material for microfluidic device to replace PDMS. Here, we introduce a practical protocol for the fabrication of NOA microfluidic devices via a serial molding process. By using indirect molding of NOA on positive-pattered wafers, we were able to prevent damage to the wafers and reduce fabrication cost of wafers. We test the performance of NOA devices in oil-in-water droplet production using organic solvents and confirmed that the devices fabricated by step-wise replica molding showed same performance compared with the devices fabricated with other methods.