참치지느러미 추출물에 의한 암세포 독성 및 Quinone Reductase 활성 증가 효과 = Cytotoxicity and Quinone Reductase Activity Stimulating Effects of Fin of Thunnus Thynnus Extracts in Various Cancer Cells

다국어 초록 (Multilingual Abstract)

In this study, we investigated the anticancer activity of the fin of Thunnus Thynnus (TT). TT was extracted with methanol (TTM), and then further fractionated into four subfractions by using solvent partition method, affording hexane (TTMH), methanol (TTMM), butanol (TTMB) and aquous (TTMA) soluble fractions. We determined the cytotoxicity of these four fractions in four kind of cancer cell lines, such as HepG2, MCF-7, B16-F10 and HT29 by MTT assay. The TTMM showed the strongest cytotoxic effect at the concentration of 150 ㎍/㎖, displaying 95% on the HepG2 cell lines and 82% on MCF-7 cell line. The morphological changes such as membrane shirinking and blebbing of cells were also observed by TTMM treatment in HT29 cell. In addition, we observed that quinone reductase (QR) activity was elevated by only TTMM and TTMH treatments in HepG2 cell. QR activity was increased to around 2.0 and 1.8 times in TTMM and TTMH treated HepG2 cell at 100 ㎍/㎖, respectively, compared to that in control. Although further studies are needed, the present work could suggest that the fin of TT has a potential to be usable as a chemo-preventive agent against cancer. (Korean J Nutrition 40(2): 147~153, 2007)

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약 및 결론
• Literature cited

참고문헌 (Reference)

1 New Eng J Med 312, "The inverse relation between fish consumption and 20- year mortality from coronary heart disease" 1205-1208, 1985

2 Park YJ, "The growth inhibitory effecs of atrina pecitinata fractions on cancer cell lines" (4) : 307-312, 2005

3 Park YJ, "The effect on antimicrobial and cytotoxicity of brassica oleracea L. fractions" (4) : 567-572, 2004

4 Kim SK, "Study on acute subcutaneous toxicity of hydroxyapatite sinter produced from tuna bone in spraguedawley rats" (2) : 97-102, 2001

5 Park BJ, "Studies on protoplast culture and fusion in cruciferae" 23-234, 1987

6 Phillipson BE, "Reduction of plasma lipoprotein and apoprotein by dietary fish oils in patients with hypertriglyceridemia" 1210-1213, 1985

7 Santamaria AB, "Quinone reductase from cells cultured in microtiter wells A screening assay for anticarcinogenic enzyme inducer" 328-336, 1988

8 Choi JS, "Properties of the ceramic composites and glass-ceramics prepared by using the natural hydroxyapatite derived from tuna bone" (3) : 394-399, 1999

9 Jung BM, "Inhibitory effects on cell surviva and quinone reductase induced activity of aster yomena fractions on human cancer cells" (1) : 8-12, 2005

10 Jung BM, "Inhibitory effects on cell surviva and quinone reductase induced activity of aster yomena fractions on human cancer cells" (1) : 8-12, 2005

11 Park SY, "Growth inhibotion effects of cancer cell lines by gloiopeltis furcata fractions in vitro" (4) : 771-775, 2005

12 Cho SG, "Food chemoprevention and immunoactivity" (1) : 56-101, 1997

13 Alley MC, "Fesaibility of drug screening with panels of human tumor cell line using a microculture tetrazolium assay" 589-601, 1988

14 Carmichael J, "Evalution of a tetrazolium baxed semiautomated colorimetric assay assessment of chemosensitivity testing" 936-942, 1987

15 Kim SK, "Evaluation of Mucous Membrane irritation by hydroxyapatite sinter produced from tuna bone in syrian hamsters" (6) : 605-609, 2000

16 Park YB, "Elucidation of anti-tumor initiator and promoter derived from seaweed-2 investigation of seaweed extracts suppressing mutagenic activity of PhIP and MeIQx" (4) : 581-586, 1998

17 Steinkellner HS, "Effects of crucifrous vegetable and their constituents on drug metabolizing enzymes involved in the bioactivation of DNA-reactive dietary carcinogens" 285-297, 2001

18 Ha YL, "Conjugated dienoic derivatices of linoleic acid" 401-407, 1981

19 Ong TM, "Chlorophyllin; a potent antimutagen against envitonmental and dietary complex mixture" 111-115, 1986

20 Carrill KK, "Biological effects of fish oils in relation to chronic diseases" 732-21 734, 1986

21 Hwang WI, "Antitumor and immunological effects of tuna extract" 353-366, 1992

22 Ryu BH, "Antitumor activity of seaweeds toward sarcoma-180" 595-600, 1989

23 Hwang WI, "A study on the effects of anticarcinogenic activity of tuna extract" Ltd 1987

24 Jung YH, "A study on the effects of anticarcinogenic activity of gloiopeltis tenax" (4) : 395-401, 2006

25 Jeon KH, "A study on the effects of anticarcinogenic activity of chondria crassicaulis" (7) : 503-511, 2005