건조스트레스에 의한 오이와 토마토 공정육묘의 광화학적 지표 해석 = The Assessment of Photochemical Index of Nursery Seedlings of Cucumber and Tomato under Drought Stress

다국어 초록 (Multilingual Abstract)

The purpose of this study is to analyze photochemical activity of nursery seedlings under drought stress, using chlorophyll fluorescence reaction analysis. Young nursery seedlings of tomato (Lycopersicon esculentum Mill.) and cucumber (Cucumis sativa L.), were grown under drought stress for 8 days. Analysis of chlorophyll fluorescence reaction (OJIP) and parameters, were performed to evaluate photochemical fluctuation in nursery seedlings under drought stress. Chlorophyll fluorescence reaction analysis showed maximal recorded fluorescence (P) decreased from the 5 day after treatment in tomato seedlings, while an amount of chlorophyll fluorescence increased at the J-I step. Thus, physiological activity was reduced. In cucumber seedlings, maximal recorded fluorescence (P) and maximal variable fluorescence (FV) lowered from the 4 day after treatment, and chlorophyll fluorescence intensity of J-I step increased. Chlorophyll fluorescence parameter analysis showed electron transfer efficiency of PSⅡ and PSⅠ were significantly inhibited with decreasing ET2O/RC and RE1O/RC from the 5 day after treatment, in tomato seedlings and from the 4 day after treatment, in cucumber seedlings. ET2O/RC and PIABS significantly changed. In conclusion, 6 indices such as FV/FM, DIO/RC, ET2O/RC, RE1O/RC, PIABS and PITOTALABS were selected for determining drought stress in nursery seedlings. Drought stress factor index (DFI) was used to evaluate whether the crop was healthy or not, under drought stress. Cucumber seedlings were less resistant to drought stress than tomato seedlings, in the process of drought stress.

국문 초록 (Abstract)

본 연구는 엽록소형광반응 분석을 이용하여 건조스트레스에 의한 공정육묘의 광화학적 활력을 분석하였다. 토마토와 오이 공정육묘를 8일 동안 건조스트레스 처리를 하였다. 엽록소형광반응 (OJIP)과 매개변수 분석을 통해 건조스트레스로 인한 작물의 광화학적 변동을 평가하였다. 엽록소 형광반응 (OJIP) 분석 결과, 토마토는 처리 후 5일부터 최대 형광량 (P)이 감소한 반면 J-I 단계에서는 엽록소 형광량이 증가하였다. 따라서 생리적 활력이 감소한 것을 알 수 있었다. 오이의 경우 처리 후 4일부터 최대 형광 (P) 및 변동 형광량 (FV)이 낮아지고 J-I 단계의 엽록소 형광 수치가 증가하였다. 엽록소 형광 매개변수 분석한 결과 토마토는 처리 후 5일부터 특히 ET2O/RC와 RE1O/RC가 감소하면서 광계II와 광계I의 전자전달효율이 유의적으로 낮아진 것으로 보인 반면 오이는 처리 후 4일부터 ET2O/RC와 PIABS가 상당히 변화하였다. 결론적으로 FV/FM, DIO/RC, ET2O/RC, RE1O/RC, PIABS, PITOTALABS 6개의 지표가 공정육묘의 건조스트레스를 판단하는 지표로 선정되었다. 건조스트레스지수 (DFI)를 통해 건조스트레스로 인한 작물별 건전성 평가를 하였고 오이의 경우 토마토에 비해 건조 저항성이 낮은 것으로 판단되었다.

참고문헌 (Reference)

1 김문일, "휴대용 근적외선 카메라로부터 얻어진 DI(Detection Index)를 이용한 소나무 재선충 피해목의 조기감별" 한국산림과학회 100 (100): 374-381, 2011

2 박소현, "옥수수의 파종시기 및 질소수준별 광화학적 반응 해석" 한국작물학회 60 (60): 1-7, 2015

3 여욱현, "스마트팜 구현을 위한 연구동향 및 ICT 핵심기술 분석" (사) 한국생물환경조절학회 25 (25): 30-41, 2016

4 손민호, "공간통계학적 방법에 의한 소나무 재선충 피해의 자연적 확산유형분석" 한국산림과학회 95 (95): 240-249, 2006

5 유성녕, "고추의 엽록소 형광 이미지 분석법에 의한 한발스트레스 지표화 가능성" 한국토양비료학회 45 (45): 676-682, 2012

6 Genty B, "The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence" 990 : 87-92, 1989

7 Calatayud A, "Spatial-temporal variations in rose leaves under water stress conditions studied by chlorophyll fluorescence imaging" 44 : 564-573, 2006

8 Govindjee G, "Sixty-three years since Kautsky: chlorophyll a fluorescence" 22 : 131-160, 1995

9 Massacci A, "Response of the photosynthetic apparatus of cotton (Gossypium hirsutum)to the onset of drought stress under field conditions studied by gas-exchange analysis and chlorophyll fluorescence imaging" 46 : 189-195, 2008

10 RDA, "Progress plant for korean smart-farm based on ICT convergence" Rural Development of Administration 1-51, 2015

11 Oukarroum A, "Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering" 60 : 438-446, 2007

12 Fracheboud Y, "Practical applications of chlorophyll fluorescence in plant biology" Springer 125-150, 2003

13 Fghire R, "Physiological and photosynthetic response of quinoa to drought stress" 75 : 174-183, 2015

14 Björkman O, "Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins" 170 : 489-504, 1987

15 유성녕, "Photochemical Response Analysis on Drought Stress for Red Pepper (Capsiumannuum L.)" 한국토양비료학회 46 (46): 659-664, 2013

16 Živčák M, "Performance index as a sensitive indicator of water stress in Triticum aestivum L. L" 54 : 133-139, 2008

17 Stirbet A, "On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and photosystem II: basics and applications of the OJIP fluorescence transient" 104 : 236-257, 2011

18 Thach W, "On the mechanism of cerebellar contributions to cognition" 6 : 163-167, 2007

19 Kautsky H, "Neue versuche zur kohlensäureassimilation" 19 : 964-, 1931

20 Paillotin G, "Movement of excitations in the photosynthetic domains of photosystem II" 58 : 237-252, 1976

21 Butler W, "Fluorescence quenching in photosystem II of chloroplasts" 376 : 116-125, 1975

22 Burke JJ, "Evaluation of source leaf responses to waterdeficit stresses in cotton using a novel stress bioassay" 143 : 108-121, 2007

23 Bandaru V, "Evaluating leaf and canopy reflectance of stressed rice plants to monitor arsenic contamination" 13 : 606-, 2016

24 Okçu G, "Effects of salt and drought stresses on germination and seedling growth of pea (Pisum sativum L.)" 29 : 237-242, 2005

25 Falqueto AR, "Effects of drought stress on chlorophyll a fluorescence in two rubber tree clones" 224 : 238-243, 2017

26 Okunlola GO, "Effect of water stress on the growth and some yield parameters of Solanum lycopersicum L" 9 : 1755-1761, 2015

27 Oukarroum A, "Drought stress effects on photosystem I content and photosystem II thermotolerance analyzed using Chl a fluorescence kinetics in barley varieties differing in their drought tolerance" 137 : 188-199, 2009

28 Jefferies R, "Drought and chlorophyll fluorescence in fieldgrown potato (Solanum tuberosum)" 90 : 93-97, 1994

29 Strasser RJ, "Dissipative Strukturen als thermodynamischer Regelkreis des Photosyntheseapparates" 98 : 53-72, 1985

30 Schuerger AC, "Comparison of two hyperspectral imaging and two laser-induced fluorescence instruments for the detection of zinc stress and chlorophyll concentration in bahia grass (Paspalum notatum Flugge.)" 84 : 572-588, 2003

31 Lichtenthaler HK, "Chlorophyll fluorescence imaging of photosynthetic activity with the flash-lamp fluorescence imaging system" 43 : 355-369, 2005

32 Govindjee G, "Chlorophyll a fluorescence: a signature of photosynthesis" Springer 1-42, 2004

33 Van Rensburg L, "Can screening criteria for drought resistance in Nicotiana tabacum L. be derived from the polyphasic rise of the chlorophyll a fluorescence transient (OJIP)?" 62 : 337-341, 1996

34 Baker NR, "Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities" 55 : 1607-1621, 2004

35 Strasser RJ, "Advanced techniques in soil microbiology" Springer 319-341, 2007

36 Park KH, "A study on the current state and development strategies of raising seedling industry" Korea Rural Economic Institute 2011