Effects of Astragalus membranaceus on Angiogenesis

다국어 초록 (Multilingual Abstract)

Objectives : The aim of this study is to identify and characterize whether Astragalus membranaceus (AM) could induce angiogenesis in vitro and in vivo.
Therefore, this study may allow better understanding of the relationship between angiogenesis and wound healing, and ischemia control by AM in oriental medicines and be the basement of developing herbal acupuncture for treatment of wound healing, ischemia and bone fracture.

Methods : This study investigated the effects on angeogenesis of AM using human umbilical vein endothelial cells (HUVECs) and Matrigel angiogenesis model.

Results : AM significantly increased HUVECs proliferation in a dose-dependent manner. In addition, AM increased migration and tube-like formation in HUVECs. The expression of basic fibroblast growth factor (bFGF), an angiogenesis-stimulating growth factor, was dose-dependently increased by AM. The angiogenic activity of AM was confirmed using an in vivo Matrigel angiogenesis model, showing promotion of blood vessel formation.

국문 초록 (Abstract)

목적 : 황기가 혈관 신생 작용이 있는지에 관하여 관찰한다. 황기는 상처의 치유나 허혈성 질환에 효과를 나타내는 것으로 알려져 있다. 이러한 효과가 황기의 혈관 신생 작용과의 관련성을 이해하며 향후 임상에 쓰일 수 있는 황기 약침액 개발을 위한 기초 자료를 목표로 한다.

방법 : 황기의 혈관 신생 작용의 관찰을 위하여 human umbilical vein endothelial cells (HUVECs)와 Matrigel angiogenesis model 을 이용하여 연구하였다.

결과 : 황기는 용량에 따라서 HUVECs의 증식을 나타내었다. 또한 혈관 내피 세포의 이동과 관형 형성을 보였다. 혈관 신생 물질인 basic fibroblast growth factor (bFGF) 가 황기에 의해 증가하였다. Matrigel angiogenesis model에서 황기는 조직학적으로 혈관 형성을 촉진하였으며, 헤모글로빈의 증가를 나타내었다.

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