오이(Cucumis sativus L.) 떡잎의 노쇠화와 관련된 유전자에 영향을 미치는 Jasmonic acid의 역할에 관한 연구 = A study about characterization of jasmonic acid to senescence-associated genes in cucumber cotyledon

다국어 초록 (Multilingual Abstract)

New plant leaf begins from leaf primordia to develop a leaf. Leaf senescence is one type of programed cell death(PCD) that is controlled by genetic program. Previously, studies identified many cucumber (Cucumis sativus L.) SAGs (senescence-associated genes). In this study, cucumber SAGs were selected to examine the gene expression patterns and to characterize of Jasmonic acid response which may affect in these genes. The SAGs were sen 4-1(pheophytinase encoding gene), sen 15-8(L-asparaginase encoding gene) and sen 053(ATPase subunitⅢ encoding gene). The most probable function of genes sequences was identified throughout NCBI-GenBank database search using tbalstx or blastx finding module. The sen 4-1 peptide sequence was matched completely with pheophytinase. The sen 15-8 peptide sequence matched in 89% with L-asparaginase. The sen 053 peptide sequence matched completely with ATPase subunitⅢ. Jasmonic acid was treated at the 9th day of after seeds sowing and then cucumber cotyledons were collected after 3 days passed of phytohormone treatment. Therefore, cotyledons were detached at the 14th, 21th, 28th day after seeds sowing and senescence stage Ⅰ(50% yellow), stage Ⅱ(70% yellow) until 45 days of cucumber development. We examined the activity of the genes by RT-PCR approach. As a result of the experiment, Sen 4-1 gene and Sen 053 genes were repressed by jasmonic acid. Sen 15-8 gene did not show any effect of jasmonic acid treatment.