Enhanced tolerance to heat stress in transgenic tomato seeds and seedlings overexpressing a trehalose-6-phosphate synthase/ phosphatase fusion gene

다국어 초록 (Multilingual Abstract)

A fusion gene coding for trehalose-6-phosphate synthase/phosphatase (TPSP) derived from Escherichia coli was introduced into tomato plants (Solanum lycopersicum cv. Joyful) to generate overexpressing lines and enhance the level of trehalose in the seeds. Trehalose has been implicated in tolerance to abiotic stress in plants, so the response to heat stress of seeds from the transgenic lines and seeds from wild-type plants were compared. Wild-type heat-treated seeds had a germination rate of 17%, while four transgenic lines had germination rates over 50% following heat stress. In one heat-treated transgenic line, 100% of the seeds germinated, and therefore had a germination rate six times that of seeds from wild-type plants. Moreover, quantitative PCR revealed that the expression of diverse genes that respond to heat stress was enhanced in TPSP transgenic seeds compared to wild-type seeds 150 min after the onset of heat stress. The enhanced germination rate and expression of these genes in the transgenic seeds were essentially mimicked in wild-type seeds treated with 1 mM exogenous trehalose.
Therefore, accumulated trehalose and associated metabolites may act as signaling molecules that enhance the expression of heat stress-responsive genes and confer heat-stress tolerance to seeds.

참고문헌 (Reference)

1 Delorge I, "Trehalose-6-phosphate synthase 1 is not the only active TPS in Arabidopsis thaliana" 466 (466): 283-290, 2015

2 Li H, "Trehalose protects wine yeast against oxidation under thermal stress" 26 : 969-976, 2010

3 Doehlemann G, "Trehalose metabolism is important for heat stress tolerance and spore germination of Botrytis cinerea" 152 : 2625-2634, 2006

4 Müller J, "Trehalose metabolism in sugar sensing and plant development" 147 : 37-47, 1999

5 Goddijn OJM, "Trehalose metabolism in plants" 4 : 315-319, 1999

6 Vogel G, "Trehalose metabolism in Arabidopsis : occurrence of trehalose and molecular cloning and characterization of trehalose-6-phosphate synthase homologues" 52 : 1817-1826, 2001

7 Paul MJ, "Trehalose metabolism and signaling" 59 : 417-441, 2008

8 Iordachescu M, "Trehalose biosynthesis in response to abiotic stresses" 50 (50): 1223-1229, 2008

9 Frank G, "Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response" 60 : 3891-3908, 2009

10 Avonce N, "The Arabidopsis trehalose-6-p synthase AtTPS1 gene is a regulator of glucose, abscisic acid, and stress signaling" 136 : 3649-3659, 2004

11 Chan-Schaminet KY, "Specific interaction between tomato HsfA1 and HsfA2 creates hetero-oligomeric superactivator complexes for synergistic activation of heat stress gene expression" 284 : 20848-20857, 2009

12 Wang W, "Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response" 9 : 244-252, 2004

13 Schoffl F, "Regulation of the heat-shock response" 117 : 1135-1141, 1998

14 Morimoto RI, "Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators" 12 : 3788-3796, 1998

15 Wahl V, "Regulation of flowering by trehalose-6-phosphate signaling in Arabidopsis thaliana" 339 : 704-707, 2013

16 Al-Whaibi MH, "Plant heat-shock proteins : a mini review" 23 : 139-150, 2011

17 Young JC, "Pathways of chaperone-mediated protein folding in the cytosol" 5 : 781-791, 2004

18 Li HW, "Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice" 234 : 1007-1018, 2011

19 Ge L-F, "Overexpression of the trehalose-6-phosphate phosphatase gene OsTPP1 confers stress tolerance in rice and results in the activation of stress responsive genes" 228 : 191-201, 2008

20 Lyu JI, "Overexpression of a trehalose-6-phosphate synthase/phosphatase fusion gene enhances tolerance and photosynthesis during drought and salt stress without growth aberrations in tomato" 112 : 257-262, 2013

21 Eyles SJ, "Nature’s molecular sponges : small heat shock proteins grow into their chaperone roles" 107 : 2727-2728, 2010

22 Mishra SK, "In the complex family of heat stress transcription factors, HsfA1 has a unique role as master regulator of thermotolerance in tomato" 16 : 1555-1567, 2002

23 Prieto-Dapena P, "Improved resistance to controlled deterioration in transgenic seeds" 142 : 1102-1112, 2006

24 Karim S, "Improved drought tolerance without undesired side effects in transgenic plants producing trehalose" 64 : 371-386, 2007

25 Li S, "Glycinebetaine enhances the tolerance of tomato plants to high temperature during germination of seeds and growth of seedlings" 34 : 1931-1943, 2011

26 Nuccio ML, "Expression of trehalose-6-phosphate phosphatase in maize ears improves yield in well-watered and drought conditions" 33 : 862-869, 2015

27 Luo Y, "Exogenously-supplied trehalose protects thylakoid membranes of winter wheat from heat-induced damage" 54 : 495-501, 2010

28 Bae H, "Exogenous trehalose alters Arabidopsis transcripts involved in cell wall modification, abiotic stress, nitrogen metabolism, and plant defense" 125 : 114-126, 2005

29 Suárez R, "Enhanced tolerance to multiple abiotic stresses in transgenic alfalfa accumulating trehalose" 49 : 1791-1799, 2009

30 Ibrahim HA, "Effect of maltose and trehalose on growth, yield and some biochemical components of wheat plant under water stress" 61 : 267-274, 2016

31 Kotak S, "Complexity of the heat stress response in plants" 10 : 310-316, 2007

32 Diamant S, "Chemical chaperones regulate molecular chaperones in vitro and in cells under combined salt and heat stresses" 276 : 39586-39591, 2001

33 Seo HS, "Characterization of a bifunctional enzyme fusion of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Escherichia coli" 66 : 2484-2490, 2000

34 Gómez LD, "AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells" 64 : 1-13, 2010

35 Dijken AJH, "Arabidopsis trehalose-6-phosphate synthase 1 is essential for normal vegetative growth and transition to flowering" 135 : 969-977, 2004

36 Nover L, "Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?" 6 : 177-189, 2001

37 Leyman B, "An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana" 6 (6): 510-513, 2001

38 Debast S, "Altering trehalose-6-phosphate content in transgenic potato tubers affects tuber growth and alters responsiveness to hormones during sprouting" 156 : 1754-1771, 2011

39 Mark C. F. R. Redillas, "Accumulation of trehalose increases soluble sugar contents in rice plants conferring tolerance to drought and salt stress" 한국식물생명공학회 6 (6): 89-96, 2012

40 Satoh-Nagasawa N, "A trehalose metabolic enzyme controls inflorescence architecture in maize" 441 : 227-230, 2006

41 Murashige T, "A revised medium for rapid growth and bio assays with tobacco tissue culture" 15 : 473-497, 1962

42 Park SH, "A comparative study on the protective role of trehalose and LEA proteins against abiotic stresses in transgenic Chinese cabbage(Brassica campestris)overexpressing CaLEA or otsA" 46 : 277-, 2003

43 Miranda J, "A bifunctional TPS–TPP enzyme from yeast confers tolerance to multiple and extreme abiotic-stress conditions in transgenic Arabidopsis" 226 : 1411-1421, 2007